紫杉醇长循环纳米胶束联合vMIP-ⅡN端肽逆转乳腺癌细胞耐药的研究

董正远; 许河南; 王文锐; 杨清玲; 陈昌杰

doi:10.13898/j.cnki.issn.1000-2200.2020.09.001

紫杉醇长循环纳米胶束联合vMIP-ⅡN端肽逆转乳腺癌细胞耐药的研究

Study on the paclitaxel long-circulating nanomicelles combined with NT21MP to reverse drug resistance in breast cancer MCF-7/PR cells

摘要

摘要:
目的探讨紫杉醇长循环纳米载药胶束联合病毒巨噬细胞炎性蛋白Ⅱ（vMIP-Ⅱ）N端肽（NT21MP）对乳腺癌紫杉醇耐药细胞株（MCF-7/PR）耐药性的影响。
方法采用磺酰罗丹明B（SRB）染色法检测乳腺癌耐药细胞株MCF-7/PR的存活率；细胞划痕及Transwell实验检测紫杉醇纳米载药胶束联合NT21MP对乳腺癌耐药细胞迁移、侵袭能力的影响；流式细胞术分析细胞凋亡、周期；Western blotting实验检测细胞凋亡、耐药及上皮间充质转化相关蛋白水平表达。
结果相对于紫杉醇组，紫杉醇纳米胶束更有效地抑制细胞增殖、迁移和侵袭，凋亡蛋白Bax、caspase3的蛋白表达水平上调，抗凋亡蛋白Bcl-2表达水平下调，且耐药相关蛋白MRP、P-gp和EMT相关蛋白Vimentin、Slug表达水平下调，E-cadherin表达上调（P < 0.01）；相对于紫杉醇纳米胶束组，紫杉醇纳米胶束与NT21MP联合组对细胞增殖、迁移、侵袭、细胞周期的抑制，以及抗凋亡和逆转耐药作用更明显（P < 0.01）。
结论紫杉醇纳米胶束可有效发挥逆转乳腺癌细胞耐药性的作用，且以紫杉醇纳米胶束联合多肽药物NT21MP作用更明显。

Abstract:
ObjectiveTo investigate the effects of paclitaxel long-circulating nanomicelles combined with vMIP-Ⅱ-N-terminal peptide(NT21MP) on drug resistance of paclitaxel-resistant brest cancer MCF-7/PR cells.
MethodsSulforhodamine B(SRB) staining was used to detect the survival rate of breast cancer paclitaxel-resistant cell line MCF-7/PR.Cell scratch and Transwell assay were used to detect the effects of paclitaxel nanomicelles combined with NT21MP on the migration and invasion of paclitaxel-resistant cells.Flow cytometry was used to analyze the apoptosis and cell cycle.Western blotting was used to detect the expression of apoptosis, drug resistance and EMT-related proteins.
ResultsCompared with paclitaxel group, the paclitaxel nanomicelles could effectively inhibit the cell proliferation, migration and invasion, the expression levels of apoptotic proteins Bax and caspase3 were up-regulated, the expression levels of anti-apoptotic proteins Bcl-2 were down-regulated, the expression levels of MRP, P-GP and EMT-related proteins Vimentin and Slug were down-regulated, and the expression levels of E-cadherin were up-regulated(P < 0.01).Compared with the paclitaxel nanomicelles group, the paclitaxel nanomicelles combined with NT21MP group had more significant effects on the inhibition of cell proliferation, migration, invasion, cycle, anti-apoptosis and reversal paclitaxel resistance.
ConclusionsThe paclitaxel nanomicelles can effectively reverse the drug resistance of paclitaxel-resistant breast cancer cells, and the effects of the combination of paclitaxel manomicelles and NT21MP is more significant.

HTML全文

参考文献(16)

施引文献

资源附件(0)