白细胞介素34对大鼠根尖牙乳头干细胞增殖的影响

王旋宇; 朱永娜; 薛魁; 武庆华; 姜丽娜; 朱晨晨; 张晓东

doi:10.13898/j.cnki.issn.1000-2200.2020.12.002

白细胞介素34对大鼠根尖牙乳头干细胞增殖的影响

Effect of interleukin-34 on the proliferation of rat stem cells from the apical papilla

摘要

摘要:
目的探究炎性因子白细胞介素34（interleukin-34，IL-34）对大鼠根尖牙乳头干细胞（stem cells from rat apical papilla，SCAP）增殖的影响。
方法采用酶消化法分离培养大鼠根尖牙乳头干细胞，流式细胞仪鉴定SCAP表面标记分子，茜素红染色鉴定SCAP成骨分化潜能。将细胞分为实验组（含有不同浓度IL-34的细胞培养液）和对照组（不含IL-34的细胞培养液），四甲基偶氮唑蓝（MTT）实验检测IL-34对SCAP增殖能力的影响。
结果MTT实验结果显示，25 ng/mL、50 ng/mL、100 ng/mL IL-34实验组促进SCAP增殖，尤以50 ng/mL IL-34实验组对SCAP增殖的促进作用最显著，而200 ng/mL、400 ng/mL、600 ng/mL IL-34实验组则抑制SCAP增殖。
结论IL-34对SCAP的增殖有一定的调节作用，低浓度的IL-34促进SCAP增殖，高浓度的IL-34抑制SCAP增殖。

Abstract:
ObjectiveTo investigate the effects of interleukin-34(IL-34)on the proliferation of rat stem cells from the apical papilla(SCAP).
MethodsThe rat SCAP were isolated and cultured using enzyme digestion method.The surface marking molecules of SCAP were identified using flow cytometry, and the Alizarin red staining was used to identify the osteogenic potential of SCAP.The cells were divided into the experimental group(cell culture medium with different concentrations of IL-34)and control group(cell culture medium without IL-34).The effects of IL-34 on the proliferation of SCAP were detected using MTT assay.
ResultsThe results of MTT assay showed that the proliferation of SCAP was promoted at the concentration of 25 ng/mL, 50 ng/mL and 100 ng/mL of IL-34, especially the 50 ng/mL of IL-34.The proliferation of SCAP was inhibited at the concentration of 200 ng/mL, 400 ng/mL and 600 ng/mL of IL-34.
ConclusionsIL-34 can regulate the proliferation of SCAP.The low concentration of IL-34 can promote the proliferation of SCAP, while the high concentration of IL-34 can inhibit the proliferation of SCAP.

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