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    全反式维A酸对肺腺癌H1299细胞放射敏感性研究

    Effect of all-trans retinoic acid on radiosensitivity of lung adenocarcinoma H1299 cells

      摘要
    • 摘要:
      目的探讨全反式维A酸（ATRA）对肺腺癌细胞株H1299放射敏感性影响及其分子机制。
      方法MTT法检测ATRA对H1299细胞存活率的影响；平板克隆形成实验检测H1299细胞的放射敏感性；流式细胞术检测细胞周期；Western blotting检测survivin与NF-κB的蛋白表达情况。
      结果不同浓度ATRA对H1299细胞均有抑制作用，浓度为10 μmol/L时最佳（P < 0.05）。相对单独ATRA处理，10 μmol/L ATRA联合不同剂量的射线照射后，细胞生长抑制率明显增加（P < 0.01）。ATRA作用和射线照射后的细胞凋亡增多（P < 0.01），ATRA联合射线照射作用后的细胞总凋亡率明显高于单纯射线照射（P < 0.01）。与对照组、放射组、ATRA组相比，ATRA+放射组G0/G1期比例明显增加（P < 0.01）。与放射组相比，ATRA+放射组的细胞存活分数值降低，ATRA可以增加肺腺癌H1299细胞放射敏感性，增敏比为1.406。Western blotting结果显示，ATRA+放射组细胞survivin、NF-κB蛋白表达明显降低（P < 0.01）。
      结论ATRA对肺腺癌H1299细胞具有放射增敏作用，其机制可能与ATRA直接抑制H1299细胞增殖、促进H1299细胞凋亡，下调survivin及NF-κB蛋白表达有关。

       

      Abstract:
      ObjectiveTo investigate the effects of all-trans retinoic acid(ATRA) on the radiosensitivity of lung adenocarcinoma cell line H1299, and explore its possible molecular mechanisms.
      MethodsMTT assay was used to determine the effects of ATRA on the survival rate of H1299 cells, the radiosensitivity of H1299 cells was detected using plate cloning formation experiment, the H1299 cell cycle was detected using flow cytometry and the protein expression levels of survivin and NF-κB in the cells were detected using Western blotting.
      ResultsThe H1299 cells could be inhibited by ATRA at different concentrations, and the best concentration of which was 10 μmol/L(P < 0.05).Compared with the cells treated with ATRA alone, 10 μmol/L ATRA combined with different doses of radiation, the inhibition rate of cell growth significantly increased(P < 0.01), the number of cell apoptosiss increased(P < 0.01), and the total apoptosis rate of ATRA combined with radiation was significantly higher than that of ATRA alone(P < 0.01).Compared with the control group, radiation group, and ATRA group, the proportion of G0/G1 phase in ATRA combined with radiation group significantly increased(P < 0.01).Compared with the radiation group, the cell survival fraction in ATRA combined with radiation group decreased, and ATRA could increase the radiosensitivity of lung adenocarcinoma H1299 cells with a sensitization ratio of 1.406.The results of Western blotting showed that the expression levels of survivin and NF-κB in H1299 cells significantly decreased in ATRA combined with radiation group(P < 0.01).
      ConclusionsATRA can increase the radiosensitivity of lung adenocarcinoma H1299 cells, and the mechanism may be related to ATRA directly inhibiting the proliferation of H1299 cells, promoting the apoptosis of H1299 cells and down-regulating the survivin protein and NF-κB protein expression.

       

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