梅毒螺旋体感染者血清Tim-3水平与机体免疫功能相关性分析

刁玉增; 安仲武; 李海英; 程良碧; 谈笑

doi:10.13898/j.cnki.issn.1000-2200.2021.03.021

梅毒螺旋体感染者血清Tim-3水平与机体免疫功能相关性分析

Analysis on the relationship between the serum level of Tim-3 and immune function in treponemia pallidum infection patients

摘要

摘要:
目的分析梅毒螺旋体感染者血清T淋巴细胞免疫球蛋白黏蛋白分子-3（Tim-3）水平，探讨其与机体免疫功能的相关性。
方法选择梅毒螺旋体感染者105例，其中一期梅毒病人26例、二期梅毒病人27例、三期梅毒病人21例、隐性梅毒病人31例，另选择30名健康体检者作为对照组，检测各组血清Tim-3水平、体液免疫功能指标及T淋巴细胞亚群水平，并检测活动期梅毒治疗后血清Tim-3水平变化。
结果梅毒组病人血清Tim-3、IgG、IgM、IgA水平明显高于对照组（P < 0.01）；二期、三期梅毒组Tim-3水平高于一期梅毒组和隐性组（P < 0.05~P < 0.01）。梅毒组病人CD4⁺ T细胞水平、CD4⁺/CD8⁺值均明显低于对照组（P < 0.01），CD8⁺ T细胞水平明显高于对照组（P < 0.01）。活动期梅毒经正规驱梅治疗后，血清Tim-3水平和外周血CD8⁺ T细胞水平明显低于治疗前（P < 0.01），外周血CD4⁺ T细胞水平、CD4⁺/CD8⁺值均明显高于治疗前（P < 0.01）。Pearson相关分析显示，梅毒螺旋体感染者Tim-3水平与CD8⁺ T细胞水平呈正相关关系（r=0.52，P < 0.05），与CD4⁺ T细胞水平呈负相关关系（r=-0.46，P < 0.05），与IgG、IgM、IgA无明显相关关系（P>0.05）。
结论梅毒螺旋体感染者存在Tim-3水平的升高、体液免疫功能的亢进及细胞免疫功能的失衡，血清Tim-3水平与CD4⁺、CD8⁺ T细胞水平存在相关性，可能参与了梅毒细胞免疫功能紊乱的过程，加速了病程进展。

Abstract:
ObjectiveTo analyze the serum level of T-cell immunoglobulin domain and mucin domain-containing molecule 3(Tim-3) in treponemia pallidum infection patients, and explore its correlation with immune function.
MethodsOne hundred and five patients with ponemia pallidum infection included 26 cases of primary syphilis, 27 cases of secondary syphilis, 21 cases of third syphilis and 31 cases of latent syphilis, and 30 healthy subjects were set as the control group.The serum levels of Tim-3, humoral immunity index and T lymphocyte subgroup in all subjects were detected.The serum level of Tim-3 in active syphilis patients were detected after treatment.
ResultsThe serum levels of Tim-3, IgG, IgM and IgA in treponemia pallidum infection groups were higher than those in control group(P < 0.01), and the serum levels of Tim-3 in secondary and third syphilis groups were significantly higher than those in primary and latent syphilis groups(P < 0.05 to P < 0.01).The CD4⁺T cell level and ratio of CD4⁺/CD8⁺ in patients with syphilis were significantly lower than those in control group(P < 0.01), while the CD8⁺T cell level in patients with syphilis was significantly higher than that in control group(P < 0.01).After regular syphilis depravation treatment for active syphilis, the serum levels of Tim-3 and peripheral blood CD8⁺ T cell were significantly lower than those before treatment(P < 0.01), and the peripheral blood CD4⁺ T cell level and CD4⁺/CD8⁺ value were significantly higher than those before treatment(P < 0.01).The results of Pearson correlation analysis showed that the level of Tim-3 was positively correlated with the level of CD8⁺ T cells(r=0.52, P < 0.05), negatively correlated with level of CD4⁺ T cells(r=-0.46, P < 0.05), but not significantly correlated with IgG, IgM and IgA(P>0.05).
ConclusionsThe Tim-3 level increasing, hyperhumoral immune function and imbalance of cellular immune function exist in the patients infected with treponema pallidum.The serum level of Tim-3 is correlated with the CD4⁺ and CD8⁺ T cell levels, which may be involved in the process of cellular immune dysfunction of syphilis and accelerating the course of the disease.

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