调节性B细胞在卵巢癌病人外周血中表达及其临床意义

衡燕春; 马珊珊; 马玲

doi:10.13898/j.cnki.issn.1000-2200.2021.06.015

调节性B细胞在卵巢癌病人外周血中表达及其临床意义

Clinical significance of regulatory B cells in peripheral blood of ovarian cancer

摘要

摘要:
目的探究调节性B细胞(Bregs)在卵巢癌病人外周血中的表达频率及其与FIGO分期间的关系，并分析其与调节性T细胞(Tregs)及IL-10之间的相关性。
方法将卵巢癌(23例)和卵巢良性肿瘤病人(26例)纳入研究，流式细胞术检测外周血CD19⁺CD24^hiCD38^hiBreg和CD4⁺CD25⁺Treg细胞表达频率及血清IL-10表达水平。并分析两种细胞与FIGO分期、IL-10浓度的关联及两者间的相关性。
结果卵巢癌病人外周血中Bregs、Tregs百分比高于卵巢良性肿瘤病人(P < 0.01)，并与FIGO分期密切相关，卵巢癌Ⅲ期+Ⅳ期病人高于Ⅰ期+Ⅱ期(P < 0.01)；卵巢癌病人血清IL-10高于卵巢良性肿瘤病人(P < 0.01)。相关性分析显示：卵巢癌病人外周血Breg细胞与Treg细胞比例呈正相关关系(P < 0.05)；卵巢癌病人血清IL-10与Bregs、Tregs细胞均呈正相关关系(P < 0.05)。
结论Bregs和Tregs在卵巢癌病人外周血中所占比例显著升高，能够通过分泌IL-10参与卵巢癌免疫反应，这可能为卵巢癌的免疫治疗提供新靶点。

Abstract:
ObjectiveTo explore the expression frequency of regulatory B cells(Bregs) in peripheral blood of ovarian cancer patients and its relationship with FIGO staging, and analyze its correlation with regulatory T cells(Tregs) and interleukin-10(IL-10).
MethodsThe ovarian cancer(23 cases) and benign ovarian tumor patients(26 cases) were investigated.The expression frequency of CD19⁺CD24^hiCD38^hiBreg and CD4⁺CD25⁺Treg cells in peripheral blood and expression levels of serum IL-10 in all cases were detected using flow cytometry.The correlation between the two kinds of cells and FIGO staging, IL-10 concentration, and the correlation between FIGO staging and IL-10 concentration were analyzed.
ResultsThe percentages of Bregs and Tregs in peripheral blood of ovarian cancer patients were higher than those of benign ovarian tumor patients(P < 0.01), and closely related to FIGO stage.The percentages of Bregs and Tregs in peripheral blood of patients with stage Ⅲ+Ⅳovarian cancer were higher than those of patients with stageⅠ+Ⅱovarian cancer(P < 0.01).The serum levels of IL-10 in ovarian cancer patients were significantly higher than that in benign ovarian tumor patients(P < 0.01).The results of correlation analysis showed that the proportion of Bregs in peripheral blood of ovarian cancer patients was positively correlated with the proportion of Tregs(P < 0.05).The serum level of IL-10 was positively correlated with Breg and Treg cells in ovarian cancer patients(P < 0.05).
ConclusionsThe proportion of Bregs and Tregs in the peripheral blood of ovarian cancer patients is significantly increased, and they can participate in the immune response of ovarian cancer through secreting IL-10, which may provide a new target for the immunotherapy of ovarian cancer.

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