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    HCV-cAg与HCV-Ab、HCV-RNA及肝功能之间的关系探讨

    Study on the relationship between HCV-cAg, and HCV-Ab, HCV-RNA and liver function

      摘要
    • 摘要:
      目的探讨丙型肝炎病毒核心抗原（hepatitis C virus core antigen，HCV-cAg）与丙型肝炎病毒RNA（hepatitis C virus RNA，HCV-RNA）关系以及丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、谷氨酰转肽酶（GGT）水平与HCV-RNA病毒载量之间的相关性。
      方法选取78例HCV抗体检测阳性的丙型肝炎或疑似丙型肝炎病人作为观察组，另选取同期健康体检者20名作为对照组。采用ELISA法和荧光定量PCR法等对2组血清样本进行HCV-cAg、HCV-RNA和ALT、AST、GGT水平测定，并比较各参数之间的关系。
      结果观察组HCV-cAg阳性检出率为41.0%（32/78），HCV-RNA阳性检出率为53.8%（42/78），差异有统计学意义（P < 0.01），且二者之间具有较好一致性（κ=0.747）。观察组ALT、AST和GGT水平均明显高于对照组（P < 0.01），但与HCV-RNA病毒载量均无明显相关性（P>0.05）。
      结论HCV-cAg和HCV-RNA在检测丙型肝炎方面有较好一致性和相关性，可为丙型肝炎的临床诊断提供有价值的依据。

       

      Abstract:
      ObjectiveTo investigate the relationship between hepatitis C virus core antigen(HCV-cAg) and hepatitis C virus RNA(HCV-RNA), and association between levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and glutamyl transpeptidase(GGT), and HCV-RNA viral load.
      MethodsSeventy-eight patients with positive HCV antibody or suspected hepatitis C were divided into the observation group, and another 20 healthy examinees were set as the control group during the same period.The serum levels of HCV-cAg, HCV-RNA, ALT, AST and GGT in two groups were determined by ELISA and fluorescence quantitative PCR, and the relationship among the above parameters were compared.
      ResultsIn the observation group, the positive detection rate of HCV-cAg was 41.0%(32/78), the positive detection rate of HCV-RNA was 53.8%(42/78), the difference of which was statistically significant(P < 0.01), and there was a good consistency between the two groups(κ=0.747).The levels of ALT, AST and GGT levels in observation group were significantly higher than those in control group(P < 0.01), but there was no significant correlation with HCV RNA viral load(P>0.05).
      ConclusionsThe HCV-cAg and HCV-RNA have good consistency and correlation in the detection of hepatitis C, and can provide valuable basis for the clinical diagnosis of hepatitis C.

       

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