高迁移率族蛋白B1在乳腺浸润性导管癌组织中的表达及其与预后的关系

华湘黔; 周建华

doi:10.13898/j.cnki.issn.1000-2200.2021.10.009

高迁移率族蛋白B1在乳腺浸润性导管癌组织中的表达及其与预后的关系

Expression of high mobility group protein B1 in breast invasive ductal carcinoma tissues and its relationship with prognosis

摘要

摘要:
目的分析高迁移率族蛋白B1(high mobility group protein B1，HMGB1)在乳腺浸润性导管癌组织中的表达及其与预后的关系。
方法收集139例女性乳腺浸润性导管癌病人的临床病理资料。采用免疫组织化学法检测乳腺浸润性导管癌组织与癌旁正常组织中HMGB1的表达水平，分析HMGB1表达与乳腺浸润性导管癌病人临床病理特征的关系，评价其与乳腺浸润性导管癌病人预后的关系。
结果HMGB1在乳腺浸润性导管癌组织中细胞核高表达率及胞质阳性率分别为80.58%与16.55%，均明显高于在癌旁正常组织中的表达46.04%与0.00%(P < 0.01)。HMGB1在组织学高级别、雌激素受体阴性、孕激素受体阴性乳腺浸润性导管癌组织中细胞质阳性率更高(P < 0.05)，细胞核高表达率在组织学高级别中更高(P < 0.05)。肿瘤组织学分级是HMGB1在乳腺浸润性导管癌组织中细胞核高表达的独立相关因素(OR=2.197，P < 0.05)。肿瘤组织学分级(OR=3.028，P < 0.01)、雌激素受体表达情况(OR=0.133，P < 0.01)及TNM分期(OR=3.817，P < 0.05)是HMGB1乳腺浸润性导管癌组织中细胞质阳性表达的独立相关因素。139例乳腺癌病人均获得完整随访，随访时间为66.5(18~75)个月，5年总生存率为88.49%(123/139)，5年无复发生存率为77.70%(108/139)。采用Kaplan-Meier法及Log-rank检验法分析不同细胞核、细胞质表达组间的五年生存率、五年无复发生存率以及生存曲线，显示HMGB1细胞核高表达影响乳腺浸润性导管癌的五年无复发生存率(P < 0.05)。
结论HMGB1细胞核高表达及细胞质阳性表达与乳腺浸润性导管癌病人多项预后不良因素密切相关，细胞核高表达对乳腺浸润性导管癌术后复发的预测有指导意义，细胞质阳性表达与肿瘤组织学分级、雌激素受体和TNM分期有关。

Abstract:
ObjectiveTo analyze the expression level of high mobility group protein B1(HMGB1) in breast invasive ductal carcinoma tissues, and its relationship with prognosis.
MethodsThe clinicopathological data of 139 female patients with breast invasive ductal carcinoma were collected.Immunohistochemistry was used to detect the expression levels of HMGB1 in breast invasive ductal carcinoma tissue and adjacent normal tissue.The relationship between HMGB1 expression level and clinicopathological characteristics in breast invasive ductal carcinoma was analyzed, and the relationship between HMGB1 expression level and prognosis in patients with breast invasive ductal carcinoma was evaluated.
ResultsThe nuclear high expression rate and cytoplasmic positive rate of HMGB1 in breast invasive ductal carcinoma tissues were 80.58% and 16.55%, respectively, which were significantly higher than those in adjacent normal tissues(46.04% and 0.00%) (P < 0.01).The cytoplasmic positive expression rates of HMGB1 in high histological grade, negative estrogen receptor and negative progesterone receptor of breast invasive ductal carcinoma tissues were higher(P < 0.05), and the nuclear expression rate in high histological grade tissue was significantly higher(P < 0.05).The tumor histological grade was an independent factor associated with high nuclear expression of HMGB1 in breast invasive ductal carcinoma(OR=2.197, P < 0.05).The tumor histological grade(OR=3.028, P < 0.01), estrogen receptor expression(OR=0.133, P < 0.01), and TNM stage(OR=3.817, P < 0.05) were the independent correlated factors for cytoplasmic positive expression of HMGB1 in breast invasive ductal carcinoma tissues.One hundred and thirty-nine breast cancer patients were followed up for 66.5(18-75) months, the overall 5-year survival rate and 5-year relapse-free survival rate were 88.49%(123/139) and 77.70%(108/139), respectively.The 5-year survival rate, 5-year relapse-free survival rate and survival curve among different nuclear and cytoplasmic expression groups were analyzed using Kaplan-Meier method and Log-rank it indicated that high expression of HMGB1 nucleus affected the 5-year relapse-free survival rate(P < 0.05).
ConclusionsThe high expression of HMGB1 in cell nucleus and cytoplasm is closely related to a number of poor prognostic factors in patients with breast invasive ductal carcinoma, and the high expression in nucleus is of guiding significance in predicting postoperative recurrence of breast invasive ductal carcinoma.The positive expression in cytoplasm is related to tumor histological grade, estrogen receptor and TNM stage.

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