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    皂角刺提取液对人鼻咽癌CNE-2细胞增殖和凋亡的影响

    Effect of the extract of Gleditsia sinensis thorns on the proliferation and apoptosis of human nasopharyngeal carcinoma CNE-2 cells

      摘要
    • 摘要:
      目的探讨皂角刺提取液对鼻咽癌CNE-2细胞增殖和凋亡的影响。
      方法体外培养人鼻咽癌CNE-2细胞，予不同的浓度皂角刺提取液作用于细胞，分别作用12、24、48 h后，通过MTT比色法检测细胞的增殖抑制情况。予不同浓度的皂角刺提取液作用于鼻咽癌CNE-2细胞48 h后，通过流式细胞仪检测细胞的凋亡情况。
      结果较高浓度皂角刺提取液组（3.2、6.4、12.8、25.6 mg/mL）作用于细胞48 h的增殖抑制率分别为（89.61±1.88）%、（96.99±0.66）%、（93.88±1.24）%、（84.15±0.86）%，均高于阴性对照组（P < 0.05）。2、2.5、3 mg/mL皂角刺提取液作用于细胞48 h后，细胞凋亡率分别为（15.61±1.21）%、（22.08±1.70）%、（37.62±2.07）%，均高于阴性对照组（P < 0.01）。
      结论皂角刺提取液可抑制鼻咽癌CNE-2细胞增殖并诱导其凋亡。

       

      Abstract:
      ObjectiveTo explore the effect of the extract of Gleditsia sinensis thorns on the proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells.
      MethodsNasopharyngeal carcinoma CNE-2 cells were cultured in vitro.The CNE-2 cells with different concentrations of the extract of Gleditsia sinensis thorns extract, Complete cell culture medium was used as the negative control group.The cell proliferation was detected by MTT assay.Treating nasopharyngeal carcinoma CNE-2 cells with different concentrations of the extract of Gleditsia sinensis thorns, Apoptosis of the cells was detected by flow cytometry.
      ResultsThe cell proliferation inhibition rates of the higher concentration groups(3.2, 6.4, 12.8, 25.6 mg/mL) were (89.61±1.88)%, (96.99±0.66)%, (93.88±1.24)%, (84.15±0.86)%, respectively, the apoptosis rate of each concentration group was significantly higher than the negative control group's(P < 0.05).After treating nasopharyngeal carcinoma CNE-2 cells with 2, 2.5, 3 mg/mL the extract of Gleditsia sinensis thorns, the apoptosis rate were(15.61±1.21)%, (22.08±1.70)%, (37.62±2.07)%, respectively, the apoptosis rate of each concentration group was significantly higher than the negative control group's(P < 0.01).
      ConclusionsThe extract of Gleditsia sinensis thorns can inhibit the proliferation and induce the apoptosis of nasopharyngeal carcinoma CNE-2 cells.

       

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