ObjectiveTo explore the value of serum miR-1225-5p in the diagnosis and prognosis of osteosarcoma.

MethodsOne hudred and four patients with osteosarcoma, 45 patients with periostitis and 50 healthy people were divided into the osteosarcoma group, periostitis group and control group, respectively.The serum expression levels of miR-1225-5p in three groups were detected using RT-PCR.The median was set as the cutoff value, the osteosarcoma group was subdivided into the high expression group and low expression group.The value of miR-1225-5p in the diagnosis of osteosarcoma was analyzed using ROC curve, Kaplan-Meier analysis and Log-Rank test were used to analyze the survival, and the COX proportional risk regression model was used to analyze the factors affecting the prognosis of patients.

ResultsThe relative expression level of serum miR-1225-5p in osteosarcoma group was lower than that in periostitis group and control group(P < 0.01).The expression of miR-1225-5p was correlated with the Enneking stage and tumor metastasis(P < 0.01 and P < 0.05), but the gender, age, tumor location, tumor diameter were not correlated with the WHO classification of osteosarcoma(P>0.05).The area under the curve of serum miR-1225-5p in the diagnosis of osteosarcoma was 0.785(95%CI: 0.625-0.934).When the relative expression of serum miR-1225-5p was 0.46, the Yoden index was the highest, and the sensitivity and specificity were 89.35% and 71.30%, respectively.The total survival time and disease-free survival time in high expression group were higher than those in low expression group(P < 0.05).The serum miR-1225-5p was an independent factor of OS and DFS in patients with osteosarcoma(P < 0.05 and P < 0.01).

ConclusionsMiR-1225-5p plays an important role in the diagnosis of osteosarcoma.The low expression level of serum miR-1225-5p is related to the poor prognosis of osteosarcoma patients.