外周血单个核细胞TLR4基因表达水平与AS、RA病情活动性的关系分析

钱臣; 徐扬; 蒋逸秋

doi:10.13898/j.cnki.issn.1000-2200.2022.02.010

外周血单个核细胞TLR4基因表达水平与AS、RA病情活动性的关系分析

Association of TLR4 gene expression in peripheral blood mononuclear cells with disease activity of AS and RA

摘要

摘要:
目的分析外周血单个核细胞TOLL样受体4（TLR4）基因表达与强直性脊柱炎（AS）、类风湿关节炎（RA）病情活动性的相关性。
方法选择AS、RA病人各40例，分别为AS组、RA组，另选取30名健康志愿者为对照组。以实时荧光定量逆转录聚合酶链反应检测外周血单核细胞TLR4 mRNA表达水平，并分析其与病情活动性、实验室指标的相关性。
结果外周血单个核细胞TLR4 mRNA表达水平AS组>RA组>对照组，差异有统计学意义（P < 0.01）。AS组、RA组中，活动期病人TLR4 mRNA表达水平显著高于稳定期病人（P < 0.01）。Pearson相关性分析显示，AS组病人外周血单个核细胞TLR4 mRNA表达与Bath强直性脊柱炎病情活动指数（BASDAI）评分及血清ESR、CRP、IL-17水平呈正相关关系（P < 0.05~P < 0.01），与RF水平、HLA-B27阳性率无相关性（P>0.05）；RA组病人外周血单个核细胞TLR4 mRNA表达与28个关节病情活动指数（DSA28）积分及血清ESR、RF、CRP、IL-17水平呈正相关关系（P < 0.05~P < 0.01）。
结论AS、RA病人外周血单个核细胞TLR4 mRNA表达明显上调，且与病情活动性及炎性指标具有相关性。

Abstract:
ObjectiveTo analyze the association of Toll-like receptor 4 (TLR4) gene expression in peripheral blood mononuclear cells with disease activity of ankylosing spondylitis (AS) and rheumatoid arthritis (RA).
MethodsForty patients with AS (AS group) and 40 patients with RA (RA group) were enrolled.Thirty healthy volunteers were selected as the control group.The expression levels of TLR4 mRNA in peripheral blood mononuclear cells were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR).The association of TLR4 with disease activity and laboratory indexes was analyzed.
ResultsThe expression of TLR4 mRNA in peripheral blood mononuclear cells of AS group, RA group and control group decreased gradually(P < 0.01).In AS group and RA group, TLR4 mRNA expression levels in active stage was significantly higher than those in stable stage(P < 0.01).Pearson correlation analysis showed that TLR4 mRNA expression in peripheral blood mononuclear cells was positively correlated with Bath ankylosing spondylitis disease activity index score and serum concentrations of ESR, CRP and IL-17(P < 0.05 to P < 0.01), but showed no association with RF concentration and HLA-B27 positive rate(P>0.05);TLR4 mRNA expression in peripheral blood mononuclear cells of patients with RA was positively correlated with DSA 28 score and serum concentrations of ESR, RF, CRP and IL-17(P < 0.05 to P < 0.01).
ConclusionsThe expression of TLR4 mRNA in peripheral blood mononuclear cells of AS and RA patients is significantly up-regulated, which is correlated with disease activity and inflammatory indexes.

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