大鼠星形胶质细胞CB1R体内外差异表达

孟宪栋; 张海锋

doi:10.13898/j.cnki.issn.1000-2200.2022.05.004

大鼠星形胶质细胞CB1R体内外差异表达

Study on the differential expression of CB1R in rat astrocytes in vivo and in vitro

摘要

摘要:
目的探讨大鼠脑在体和培养中星形胶质细胞大麻素Ⅰ型受体(cannabinoid type 1 receptor, CB1R)表达特点。
方法取3月龄大鼠脑海马切片，进行胶质纤维酸性蛋白(GFAP)和CB1R荧光免疫荧光双标染色；同时取刚出生大鼠鼠脑进行原代、传代培养，获取高纯度星形胶质细胞，行GFAP和CB1R免疫荧光双标染色。所有标本在激光共聚焦显微镜下，观察星形胶质细胞CB1R表达状况。
结果大鼠在体海马切片染色显示，CB1R呈点状分布于海马CA1区，尤其以锥体细胞层明显，GFAP标记的星形胶质细胞CB1R呈阴性表达；而培养中的GFAP标记阳性的星形胶质细胞，CB1R呈阳性表达，且主要集中在细胞核周围分布。
结论在体海马和细胞培养中的星形胶质细胞CB1R表达不同，与生理状态比较，细胞培养状态下星形胶质细胞CB1R表达存在明显改变。

Abstract:
ObjectiveTo explore the expression characteristics of astrocyte cannabinoid type 1 receptor(CB1R) in rat brain and astrocytes.
MethodsThe hippocampal sections of 3-month-old rats were taken for immunostaining with glial fibrillary acidic protein(GFAP) and CB1R.At the same time, the brains of newborn rats were taken for primary and subculture to obtain the high purity astrocytes, and the GFAP and CB1R immunofluorescence double labeled staining in cells were performed.The expression of CB1R in astrocytes of all specimens were observed under laser confocal microscope.
ResultsThe results of hippocampal section staining showed that the CB1R was spot-like and distributed in hippocampal CA1 region, especially in pyramidal cell layer, and the expression of CB1R in GFAP-labeled astrocytes was negative.The positive expression of CB1R in GFAP-positive astrocytes in culture was observed, which was mainly distributed around the nucleus.
ConclusionsThe expression of CB1R in astrocytes of hippocampus tissue and cell culture is different.Compared with the physiological state, the expression of CB1R in astrocytes in cell culture is significantly changed.

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