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    MMP-2、MMP-9及TIMP-1在多囊卵巢综合征病人种植窗口期内膜的表达及其临床意义

    Expression of MMP-2、MMP-9 and TIMP-1 in endometrium of PCOS patients during the implantation window phase and its clinical significance

      摘要
    • 摘要:
      目的 检测基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-2(MMP-2)及组织金属蛋白酶抑制物-1(TIMP-1)在多囊卵巢综合征(PCOS)病人种植窗口期子宫内膜组织中的表达情况，并探讨其临床意义。
      方法 选择接受促排卵后治疗的PCOS病人(PCOS组)12例和因男方因素不孕且月经正常前来就诊病人(对照组)15例，检测2组排卵后第7天子宫内膜组织中MMP-9、MMP-2和TIMP-1蛋白表达情况，利用病理图像分析技术，对实验结果进行半定量分析。
      结果 MMP-9、MMP-2和TIMP-1在2组子宫内膜组织中均呈阳性表达，在间质细胞和腺上皮细胞的细胞质内均有棕黄色颗粒物沉着，以腺上皮表达为主。PCOS组子宫内膜腺上皮MMP-2、MMP-9及TIMP-1表达均明显低于对照组(P < 0.01)，子宫内膜间质MMP-9表达亦低于对照组(P < 0.05)。
      结论 MMP-9、MMP-2和TIMP-1在PCOS组病人的种植窗口期子宫内膜中的表达水平较低，这可能是导致PCOS病人采用促排卵治疗后妊娠率低、流产率高的原因之一。

       

      Abstract:
      Objective To investigate the expression of matrix metalloproteinase(MMP)-9, MMP-2 and matrix metalloproteinase inhibitor-1(TIMP-1) in endometrium of polycystic ovary syndrome(PCOS) patients during the implantation window phase, and to explore its significance.
      Methods A total of 12 patients with PCOS who received post ovulation induction treatment(PCOS group) and 15 patients with infertility due to male factors and normal menstruation(control group) were selected.The expressions of MMP-9, MMP-2 and TIMP-1 proteins in endometrial tissue of the two groups on the seventh day after ovulation were detected.The pathology image analysis technology was employed to conduct the semi-quantitative analysis.
      Results The expressions of MMP-9, MMP-2 and TIMP-1 in the two groups in endometrium were positive, and the brown granule cell pigmentation was found in the glandular epithelial cells and stromal cells cytoplasm, especially in the former ones.The expressions of MMP-2, MMP-9 and TIMP-1 in endometrial glandular epithelium in PCOS group were significantly lower than those in control group(P < 0.01), and the expression of MMP-9 in endometrial stroma was also lower than that in control group(P < 0.05).
      Conclusions The low expression of MMP-9, MMP-2 and TIMP-1 in the implantation window phase of endometrium may be one of the causes of low pregnancy and high abortion rate in PCOS patients undergoing ovulation induction treatment.

       

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