高级检索
    马新壮, 周勇兵, 王力. 芦丁对高糖、高脂饮食致小鼠肾脏损害的保护作用[J]. 蚌埠医科大学学报, 2022, 47(8): 989-993. DOI: 10.13898/j.cnki.issn.1000-2200.2022.08.001
    引用本文: 马新壮, 周勇兵, 王力. 芦丁对高糖、高脂饮食致小鼠肾脏损害的保护作用[J]. 蚌埠医科大学学报, 2022, 47(8): 989-993. DOI: 10.13898/j.cnki.issn.1000-2200.2022.08.001
    shu
    MA Xin-zhuang, ZHOU Yong-bing, WANG Li. Protective effect of rutin on kidney damage induced by high sugar and fat diet in mice[J]. Journal of Bengbu Medical University, 2022, 47(8): 989-993. DOI: 10.13898/j.cnki.issn.1000-2200.2022.08.001
    Citation: MA Xin-zhuang, ZHOU Yong-bing, WANG Li. Protective effect of rutin on kidney damage induced by high sugar and fat diet in mice[J]. Journal of Bengbu Medical University, 2022, 47(8): 989-993. DOI: 10.13898/j.cnki.issn.1000-2200.2022.08.001
    shu

    芦丁对高糖、高脂饮食致小鼠肾脏损害的保护作用

    Protective effect of rutin on kidney damage induced by high sugar and fat diet in mice

      摘要
    • 摘要:
      目的研究芦丁对高糖高脂饮食导致的小鼠肾脏损害的保护作用。
      方法40只雄性ICR小鼠随机分成5组，正常饮食组(ND组)、高脂饮食组(HFD组)、芦丁保护高脂饮食组(RHFD组)、高糖饮食组(HSD组)、芦丁保护高糖饮食组(RHSD组)，每组各8只。连续喂养120 d后取肾脏组织测定脂代谢生化指标TC、TG含量，检测氧化应激生化指标SOD、GSH-Px和MDA水平，HE染色观察肾脏组织基本形态结构，PAS染色观察肾脏组织基底膜变化。
      结果芦丁能够明显减轻高糖、高脂饮食小鼠的体质量(P < 0.05)，提高肾脏组织抗氧化酶GSH-Px活性(P < 0.05)，并降低脂质过氧化产物MDA水平(P < 0.05)，以减轻氧化应激，改善肾小球上皮细胞和系膜增生，鲍曼囊壁层增厚，肾小管基膜增厚及空泡变性。
      结论芦丁可以有效改善高糖高脂饮食导致的小鼠肾脏损害，对高糖高脂饮食造成的小鼠肾脏损害具有一定的保护作用。

       

      Abstract:
      ObjectiveTo study the protective effect of rutin on kidney damage induced by high glucose and fat diet in mice.
      MethodsForty male ICR mice were randomly divided into 5 groups, including normal diet group(ND group), high fat diet group(HFD group), rutin protected high-fat diet group(RHFD group), high sugar diet group(HSD group), rutin protected high sugar diet group(RHSD group), 8 rats in each group.After 120 days of continuous feeding, the contents of biochemical indexes such as TC and TG of lipid metabolism were detected.The levels of biochemical indexes such as SOD, GSH-Px and MDA of oxidative stress were measured.The basic morphological structure of kidney tissue was observed by HE staining, and the changes of basement membrane of kidney tissue were observed by PAS staining.
      ResultsRutin could significantly reduce the body mass of mice fed with high sugar and fat diet(P < 0.05), increase the activity of antioxidant enzyme GSH-Px in kidney tissue(P < 0.05) and decrease the level of lipid peroxidation product MDA(P < 0.05) to reduce the oxidative stress, which improved the hyperplasia of glomerular epithelial cell and mesangial, the thickening of bowman capsule walland kidney tubular basement membrane, and the vacuolar degeneration of kidney tubular basement membrane.
      ConclusionsRutin can effectively improve the kidney damage caused by high sugar and fat diet in mice, which exerts a certain protective effect on the kidney damage caused by high sugar and fat diet in mice.

       

      • HTML全文
      • 参考文献(22)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回