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    刘雪, 张折折, 罗宝灵, 陈贵海, 梁冰. 胚胎期炎症暴露对中老年海马脑源性神经营养因子基因表达和认知功能的影响[J]. 蚌埠医科大学学报, 2023, 48(1): 83-86, 94. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.016
    引用本文: 刘雪, 张折折, 罗宝灵, 陈贵海, 梁冰. 胚胎期炎症暴露对中老年海马脑源性神经营养因子基因表达和认知功能的影响[J]. 蚌埠医科大学学报, 2023, 48(1): 83-86, 94. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.016
    shu
    LIU Xue, ZHANG Zhe-zhe, LUO Bao-ling, CHEN Gui-hai, LIANG Bing. Effects of embryonic inflammatory exposure on hippocampal BDNF gene expression and cognitive function in middle-aged and elderly mice[J]. Journal of Bengbu Medical University, 2023, 48(1): 83-86, 94. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.016
    Citation: LIU Xue, ZHANG Zhe-zhe, LUO Bao-ling, CHEN Gui-hai, LIANG Bing. Effects of embryonic inflammatory exposure on hippocampal BDNF gene expression and cognitive function in middle-aged and elderly mice[J]. Journal of Bengbu Medical University, 2023, 48(1): 83-86, 94. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.016
    shu

    胚胎期炎症暴露对中老年海马脑源性神经营养因子基因表达和认知功能的影响

    Effects of embryonic inflammatory exposure on hippocampal BDNF gene expression and cognitive function in middle-aged and elderly mice

      摘要
    • 摘要:
      目的探讨胚胎期炎症暴露对中老年期小鼠海马脑源性神经营养因子(BDNF)蛋白和mRNA表达水平与认知功能的影响。
      方法母鼠怀孕第15~17天腹腔注射脂多糖(LPS, 50 μg/kg)或等量的0.9%氯化钠溶液, 对应子鼠分别为LPS组和CON组(雌雄各半), 分别于3月龄、15月龄时完成Morris水迷宫任务, 用Western blotting和实时定量PCR检测海马BDNF表达水平。
      结果与3月龄CON组相比, 15月龄CON组游泳路程明显延长(P < 0.01), 靶象限游泳路程百分比和海马BDNF基因表达水平均下降(P < 0.01)。在两个年龄段, LPS组Morris水迷宫成绩及BDNF基因表达水平均比CON组差。Pearson相关分析显示, 仅15月龄CON组和LPS组BDNF基因表达水平与游泳路程呈负相关关系(P < 0.05), 与靶象限游泳路程百分比呈正相关关系(P < 0.05)。
      结论胚胎期炎症暴露可加重中老年小鼠空间学习记忆能力损害, 且可能与海马BDNF基因表达水平下降有关。

       

      Abstract:
      ObjectiveTo explore the effects of embryonic inflammatory exposure on brain-derived neurotrophic factor (BDNF) protein and mRNA expression in hippocampus and cognitive function in middle-aged and elderly mice.
      MethodsFemale rats were intraperitoneally injected with lipopolysaccharide (LPS, 50 μg/kg) or 0.9% sodium chloride solution on the fifteenth to seventeenth day of pregnancy, and the corresponding offsprings were set as LPS group and CON group, with equal male and female mice in each group.The Morris water maze task was completed at 3 and 15 months of age, and the BDNF expression level in hippocampus was detected by Western blotting and real-time quantitative PCR.
      ResultsCompared with the CON group at 3 months, the swimming distance in the CON group at 15 months was significantly longer (P < 0.01), and the percentage of swimming distance in the target quadrant and the expression level of BDNF gene in hippocampus were decreased (P < 0.01).Morris water maze scores and BDNF expression levels in the LPS group were worse than those in the CON group in the both age groups.Pearson correlation analysis showed that BDNF gene expression level was negatively correlated with swimming distance in the CON group and LPS group at 15 months of age (P < 0.05), and positively correlated with percentage of swimming distance in the target quadrant (P < 0.05).
      ConclusionsEmbryonic inflammatory exposure can aggravate the spatial learning and memory ability of middle-aged and elderly mice, which may be related to the decreased expression of BDNF gene in hippocampus.

       

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