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    王伟杰, 高琦, 孙正宇, 王聪, 丁洋洋, 黄浩东, 康品方, 唐碧. 2型糖尿病病人血清MMP9和PI3K水平与心房颤动的相关性研究[J]. 蚌埠医科大学学报, 2023, 48(1): 99-103. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.019
    引用本文: 王伟杰, 高琦, 孙正宇, 王聪, 丁洋洋, 黄浩东, 康品方, 唐碧. 2型糖尿病病人血清MMP9和PI3K水平与心房颤动的相关性研究[J]. 蚌埠医科大学学报, 2023, 48(1): 99-103. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.019
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    WANG Wei-jie, GAO Qi, SUN Zheng-yu, WANG Cong, DING Yang-yang, HUANG Hao-dong, KANG Pin-fang, TANG Bi. Relationship between serum MMP9 and PI3K levels and atrial fibrillation in type 2 diabetes[J]. Journal of Bengbu Medical University, 2023, 48(1): 99-103. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.019
    Citation: WANG Wei-jie, GAO Qi, SUN Zheng-yu, WANG Cong, DING Yang-yang, HUANG Hao-dong, KANG Pin-fang, TANG Bi. Relationship between serum MMP9 and PI3K levels and atrial fibrillation in type 2 diabetes[J]. Journal of Bengbu Medical University, 2023, 48(1): 99-103. DOI: 10.13898/j.cnki.issn.1000-2200.2023.01.019
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    2型糖尿病病人血清MMP9和PI3K水平与心房颤动的相关性研究

    Relationship between serum MMP9 and PI3K levels and atrial fibrillation in type 2 diabetes

      摘要
    • 摘要:
      目的探讨血清基质金属蛋白酶9(MMP9)和磷脂酰肌醇-3-羟激酶(PI3K)水平变化与2型糖尿病病人(T2DM)心房颤动(AF)发生、发展的关系。
      方法收集200例心电图提示AF的T2DM病人, 其中阵发性房颤(PaAF)62例, 持续性房颤(PeAF)70例, 永久性房颤(PerAF)68例。并选择90例单纯性T2DM病人作为对照组。ELISA和Western blotting法分别测定血清中MMP9、PI3K水平及蛋白表达情况, 并分别与已测得的左心房内径(LAD)进行相关性分析。纳入MMP9、PI3K和LAD行Pearson相关分析和二元logistics回归分析, 得出影响AF的因素。
      结果与对照组相比, PaAF、PeAF与PerAF组MMP9表达水平升高(P < 0.01)、PI3K表达水平降低(P < 0.01)。与PaAF组相比, PeAF组与PerAF组MMP9表达水平升高(P < 0.01)、PI3K表达水平降低(P < 0.01)。与PeAF组相比, PerAF组MMP9表达水平升高(P < 0.01)、PI3K表达水平降低(P < 0.01)。Western blotting检测显示MMP9存在上调趋势, PI3K存在下调趋势。Pearson法对MMP9、PI3K与LAD进行相关性分析, 结果显示在所有实验组中MMP9水平与LAD呈正相关关系(r=0.842, P < 0.01);PI3K与LAD呈负相关关系(r=0.868, P < 0.01)。二元logistics回归分析显示MMP9、PI3K、LAD是AF的危险因素(P < 0.05)。
      结论T2DM合并AF病人血清中MMP9表达水平明显升高, PI3K表达水平明显降低, 且与LAD有一定的相关性, MMP9、PI3K的激活与表达与T2DM病人AF的发生可能有关。

       

      Abstract:
      ObjectiveTo explore the relationship between the changes of serum matrix metalloproteinase-9(MMP9) and phosphatidylinositol-3-hydroxykinase(PI3K) levels mediated by oxidative stress and the occurrence and maintenance of atrial fibrillation (AF) in patients with type 2 diabetes mellitus(T2DM).
      MethodsTwo hundred T2DM patients with AF were collected, including 62 cases of paroxysmal atrial fibrillation (PaAF), 70 cases of persistent atrial fibrillation (PeAF) and 68 cases of permanent atrial fibrillation(PerAF).Ninety patients with simple T2DM were selected as control group.The levels of MMP9, PI3K and protein expression in serum were measured by ELISA and Western blotting methods, and the correlation with the measured LAD was analyzed.MMP9, PI3K and LAD were included in Pearson correlation analysis and binary logistics regression analysis, and the factors affecting AF were obtained by binary Logistics regression analysis.
      ResultsCompared with control group, the expression of MMP9 increased and the expression of PI3K decreased in PaAF, PeAF and PerAF groups.Compared with PaAF group, the expression of MMP9 in PeAF group and PerAF group increased and the expression of PI3K decreased (P < 0.01).Compared with PeAF group, the expression level of MMP9 in PerAF group increased and the expression level of PI3K decreased.Western blotting showed and there was an up-regulation trend in MMP9 and a down-regulation trend in PI3K.Pearson method was used to analyze the correlation between MMP9, PI3K and LAD.The results showed that in all experimental groups, the concentration of MMP9 was positively correlated with LAD (r=0.842), and PI3K was negatively correlated with LAD (r=0.868).Binary logistics regression analysis showed that MMP9, PI3K and LAD were risk factors for AF.
      ConclusionsThe expression of MMP9 in serum of patients with T2DM complicated with AF is significantly increased, while the expression of PI3K is significantly decreased, and there is a certain correlation between MMP9 and LAD.The activation and expression of MMP9 and PI3K may be related to the occurrence of AF in patients with T2DM.

       

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