槲皮素对过氧化氢诱导的人脐静脉内皮细胞AKT/mTOR信号通路及自噬的影响

宋涛; 官泽宇; 徐超; 朱二畅; 蔡瑶; 卢冉; 余朝文

doi:10.13898/j.cnki.issn.1000-2200.2023.02.001

槲皮素对过氧化氢诱导的人脐静脉内皮细胞AKT/mTOR信号通路及自噬的影响

Effect of quercetin on hydrogen peroxide-induced AKT/mTOR signaling pathway and autophagy in human umbilical vein endothelial cells

摘要

摘要:
目的分析槲皮素对人脐静脉内皮细胞自噬和增殖的影响，探讨其与AKT/mTOR信号通路调控的关系及机制。
方法培养人脐静脉内皮细胞，MTT法检测细胞活力，MDC染色法检测自噬泡水平，Western印迹检测自噬相关蛋白水平，运用3-MA研究槲皮素对自噬和细胞增殖的影响。
结果槲皮素能增加人脐静脉内皮细胞活力（P < 0.01），上调细胞中MDC染色标记的荧光颗粒，并伴随浓度的上调明显增加（P < 0.01），能促进脐静脉内皮细胞中自噬蛋白LC3 Ⅱ/LC3 Ⅰ水平表达（P < 0.01），降低p-AKT/AKT、p-mTOR/mTOR水平（P < 0.01）。与槲皮素组比较，槲皮素+3-MA组细胞活力和LC3 Ⅱ/LC3 Ⅰ均明显降低（P < 0.05），p-mTOR/mTOR明显增加（P < 0.05）。
结论槲皮素可通过抑制AKT/mTOR信号通路诱导细胞自噬来调控脐静脉内皮细胞增殖。

Abstract:
ObjectiveTo analyze the effect of quercetin on autophagy and proliferation of human umbilical vein endothelial cells and its relationship with AKT/mTOR signaling pathway.
MethodsMTT method was used to detect cell viability.MDC staining was used to detect the level of autophagy vesicles.Western blotting was used to detect the levels of autophagy-related proteins, and 3-MA was used to study the effect of quercetin on autophagy and cell proliferation.
ResultsQuercetin increased the viability of human umbilical vein endothelial cells and up-regulated the fluorescent particles labeled by MDC staining in the cells followed with the increase in concentration, which promoted the expressions of autophagy protein LC3 Ⅱ/LC3 Ⅰ in umbilical vein endothelial cells and reduce the levels of p-AKT/AKT and p-mTOR/mTOR(P < 0.01).Compared with the quercetin group, the cell viability and LC3 Ⅱ/LC3 Ⅰ of the quercetin+3-MA group were significantly reduced, and p-mTOR/mTOR was significantly increased(P < 0.05).
ConclusionsQuercetin can regulate the proliferation of umbilical vein endothelial cells by inhibiting the AKT/mTOR signaling pathway and inducing autophagy.

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