白细胞介素-35和叉头样转录因子3在大鼠根尖周炎中的表达
Expressions of interleukin-35 and foxhead box P3 in periapical periodontitis
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摘要:目的观察大鼠实验性根尖周炎中白细胞介素-35(interleukin-35, IL-35)与叉头样转录因子3(foxhead box p3, Foxp3)的动态表达情况, 探讨IL-35在根尖周炎中的作用。方法选取40只SPF级SD大鼠, 随机分为5组, 单侧下颌第一磨牙开髓进行根尖周炎造模, 分别于术后0、7、14、21、28 d各随机处死一组, 下颌骨拍摄X线片, 观察根尖周变化情况。取下颌第一磨牙根尖周组织进行苏木精-伊红染色(HE)观察其组织病理变化。实时荧光定量PCR反应(real-time qPCR, RT-qPCR)动态检测根尖周病损组织中IL-35和Foxp3 mRNA的表达情况。结果X线和HE染色显示随着牙髓暴露时间的延长, 根尖部炎症程度逐渐加重, 待髓腔开放21~28 d时炎症则趋于稳定, 从临床表现和组织病理学两方面证实了大鼠根尖周炎造模成功。RT-qPCR结果显示: IL-35和Foxp3 mRNA相对表达量在术后不同时期均呈波动性升高趋势(P < 0.01), 术后0~7 d迅速上升, 7~21 d表达量呈下降趋势(P < 0.05), 21~28 d又呈上升趋势(P < 0.05); Pearson相关分析显示: IL-35mRNA与Foxp3mRNA相对表达量呈正相关关系(r=0.609, P < 0.05)。结论IL-35在大鼠根尖周炎中的表达量与炎症程度成反比, 可能通过调控Treg的表达参与根尖周炎的发生发展。Abstract:ObjectiveTo observe the dynamic expressions of interleukin-35 (IL-35) and foxhead box p3 (Foxp3) in experimental rats with periapical periodontitis, and to explore the role of IL-35 in periapical periodontitis.MethodsForty SPF-grade SD rats were selected and randomly divided into 5 groups.The model of periapical periodontitis was established by pulpotomy of the unilateral mandibular first molar.One group was sacrificed at random on the 0, 7, 14, 21 and 28 d after operation, respectively.X-ray films were taken in the mandible to observe periapical changes.The periapical tissues of the mandibular first molars were collected and stained with hematoxylin-eosin(HE) to observe their histopathological changes.The expressions of IL-35 and Foxp3 mRNA in the periapical lesions were dynamically detected using real-time quantitative PCR (RT-qPCR).ResultsX-ray and HE staining showed that with the extension of pulp exposure time, the degree of periapical periodontitis increased gradually, and the inflammation tended to be stable after the pulp cavity was opened for 21 to 28 days.The clinical manifestations and histopathology confirmed the successful establishment of the periapical periodontitis.RT-qPCR results showed that the mRNA relative expression levels of IL-35 and Foxp3 increased in a fluctuating trend at different periods of operation (P < 0.01), increased rapidly at 0-7 days after pulp opening, decreased at 7-21 days (P < 0.05), and increased at 21-28 days (P < 0.05).Pearson correlation analysis showed that there was a positive correlation between IL-35 mRNA and Foxp3 mRNA expression (r=0.609, P < 0.05).ConclusionsThe expression level of IL-35 in periapical periodontitis is inversely proportional to the degree of inflammation, which may be involved in the occurrence and development of periapical periodontitis by regulating the expression of Treg.
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