lncRNA SNHG5和miR-132-3p在子痫前期病人胎盘组织中的表达关系研究

宋晔; 周信芳; 许咏梅; 谌福霞

doi:10.13898/j.cnki.issn.1000-2200.2023.07.003

lncRNA SNHG5和miR-132-3p在子痫前期病人胎盘组织中的表达关系研究

Study on the relationship between lncRNA SNHG5 and miR-132-3p expression in placenta tissue of preeclampsia patients

摘要

摘要:
目的观察长链非编码RNA小核仁RNA宿主基因5(lncRNA SNHG5)和miR-132-3p在子痫前期(PE)病人胎盘组织中的表达，分析二者的相关性及对PE的诊断价值。
方法选取112例PE病人作为研究对象，根据PE严重程度分为重度组58例和轻度组54例，另外选取同期健康妊娠孕妇60例作为对照组。采用实时荧光定量PCR检测胎盘组织中lncRNA SNHG5和miR-132-3p表达；Pearson相关分析观察PE病人胎盘组织中lncRNA SNHG5与miR-132-3p的相关性，及lncRNA SNHG5、miR-132-3p与临床指标的相关性；ROC曲线分析lncRNA SNHG5和miR-132-3p水平对PE的诊断价值。
结果轻度组和重度组PE病人24 h尿蛋白、收缩压以及舒张压水平均高于对照组(P < 0.05)；重度组收缩压、舒张压均高于轻度组(P < 0.05)。轻度组、重度组lncRNA SNHG5水平低于对照组(P < 0.05)，miR-132-3p水平高于对照组(P < 0.05)；重度组lncRNA SNHG5水平低于轻度组(P < 0.05)。胎盘组织中lncRNA SNHG5、miR-132-3p表达与PE病人24 h尿蛋白、收缩压和舒张压均有相关关系(P < 0.05~P < 0.01)，与年龄、孕周和体质量指数无明显相关关系(P>0.05)。PE病人胎盘组组织中lncRNA SNHG5与miR-132-3p表达呈负相关关系(r=-0.509，P < 0.05)。ROC曲线分析结果显示，胎盘组织中lncRNA SNHG5、miR-132-3p诊断PE的曲线下面积分别为0.865、0.883，截断值分别为0.93、1.44，敏感度分别为87.9%、79.3%，特异性分别为71.7%、83.3%；二者联合诊断PE的曲线下面积为0.921，敏感度和特异性分别为89.7%、81.7%。
结论PE病人胎盘组织中lncRNA SNHG5呈低表达，miR-132-3p呈高表达，且二者呈负相关关系，为PE的诊断和靶向治疗提供了新的思路。

Abstract:
ObjectiveTo observe the expression of long non-coding RNA small nucleolar RNA host gene 5 (lncRNA SNHG5) and miR-132-3p in placental tissue of preeclampsia (PE) patients, and to analyze the correlation between them, and their diagnostic value for PE.
MethodsA total of 112 patients with PE were selected as the study subjects, and divided into severe group (n=58) and mild group (n=54) according to the severity of PE.Sixty healthy pregnant women in the same period were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA SNHG5and miR-132-3p in placental tissue.Pearson correlation analysis was applied to observe the correlation between lncRNA SNHG5 and miR-132-3p in placental tissue of PE patients, and the correlation between lncRNA SNHG5, miR-132-3p and clinical indicators.ROC curve was employed to analyze the diagnostic value of lncRNA SNHG5 and miR-132-3p levels for PE.
ResultsThe level of 24 h urinary protein, systolic blood pressure and diastolic blood pressure of PE patients in the mild group and severe group was higher than that in the control group (P < 0.05), and the level of systolic blood pressure and diastolic blood pressure in the severe group was higher than that in the mild group (P < 0.05).The level of lncRNA SNHG5 in the mild group and severe group was lower than that in the control group (P < 0.05), and the level of miR-132-3p was higher than that in the control group (P < 0.05);the level of lncRNA SNHG5 in the severe group was lower than that in the mild group (P < 0.05).The expression of lncRNA SNHG5 and miR-132-3p in placental tissue was correlated with 24 h urine protein, systolic blood pressure and diastolic blood pressure of PE patients (P < 0.05 to P < 0.01), but was not correlated with age, gestational week and body mass index (P>0.05).There was a negative correlation between lncRNA SNHG5 and miR-132-3p expression in placental tissue of PE patients (r=-0.509, P < 0.05).The results of ROC curve analysis showed that the area under the curve of lncRNA SNHG5 and miR-132-3p in placental tissue in the diagnosis of PE was 0.865 and 0.883, the cutoff value was 0.93 and 1.44, the sensitivity was 87.9% and 79.3%, and the specificity was 71.7% and 83.3%, respectively; the area under the curve of the combination of lncRNA SNHG5 and miR-132-3p in the diagnosis of PE was 0.921, the sensitivity and specificity were 89.7% and 81.7%, respectively.
ConclusionsLncRNA SNHG5 is lowly expressed and miR-132-3p is highly expressed in the placenta tissue of PE patients, and they are negatively correlated, which provides new ideas for the diagnosis and targeted therapy of PE.

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