可注射GelMA水凝胶搭载CD271与PDGF修复骨缺损的实验研究

王照东; 李伟峰; 朱仲廉; 刘亚军; 徐陈; 刘翔宇; 周平辉; 吴敏; 官建中

doi:10.13898/j.cnki.issn.1000-2200.2023.10.003

可注射GelMA水凝胶搭载CD271与PDGF修复骨缺损的实验研究

Experimental study of injectable GelMA hydrogel loaded with CD271 and PDGF in bone defect repair

摘要

摘要:
目的探讨负载CD271与血小板衍生生长因子(PDGF)的可注射甲基丙烯酸明胶(GelMA)水凝胶支架在SD大鼠颅骨缺损修复中的效果。
方法利用5%的GelMA水凝胶与PDGF共同构建得到GelMA/PDGF支架，然后将CD271抗体对支架进行修饰。实验共分为GelMA、GelMA/PDGF、GelMA/ CD271、GelMA/PDGF/CD271 4组。通过水凝胶支架与雄性SD大鼠骨髓间充质干细胞(BMSCs)在体外共同培养，检测细胞的生物相容性及成骨相关蛋白的表达情况；颅骨缺损模型使用的动物为质量约350 g的SD雄性大鼠，并在骨缺损处植入水凝胶支架；大鼠颅骨在术后8周接受Micro-CT检测以评估修复效果。
结果BMSCs与负载CD271与PDGF的GelMA水凝胶支架具有良好的生物相容性，可提高成骨相关蛋白(碱性磷酸酶、Ⅰ型胶原蛋白、骨钙素和骨桥蛋白)的表达(P < 0.05)，并可促进修复体内颅骨的缺损。
结论负载CD271与PDGF的混合水凝胶支架具有良好的生物相容性，在骨组织工程中极具应用潜力，能促进BMSCs的成骨分化。

Abstract:
ObjectiveTo investigate the effect of an injectable gelatin methacrylamide (GelMA) hydrogel scaffold loaded with CD271 and PDGF in the repair of skull defects in SD rats.
MethodsThe GelMA/PDGF scaffold was jointly constructed by using a 5% GelMA hydrogel and PDGF, and then the scaffold was modified with the CD271 antibody.The experiments were divided into four groups: GelMA, GelMA/PDGF, GelMA/CD271, and GelMA/PDGF/CD271.The biocompatibility of the cells and the expression of osteogenic-related proteins were examined by co-culturing hydrogel scaffolds with male SD rat bone marrow mesenchymal stem cells (BMSCs) in vitro.The skull defect model was established in SD male rats weighing about 350 g and implanted with hydrogel scaffolds.Micro-CT of the rat skull was performed at 8 weeks after surgery to assess repair efficacy.
ResultsBMSCs and GelMA hydrogel scaffold loaded with CD271 and PDGF had good biocompatibility, could upregulate the expression of osteogenic-related proteins (alkaline phosphatase, collagen Ⅰ, osteocalcin, and osteopontin), and promoted the repair of cranial defects in vivo.
ConclusionsMixed hydrogel scaffold loading CD271 and PDGF has good biocompatibility and promotes the osteogenic differentiation of BMSCs, and has great application potential in bone tissue engineering.

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