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    刘旋, 张磊, 张凤军. lncRNA Zeb1-AS1调控JAK2/STAT3信号通路影响骨关节炎软骨细胞的增殖和凋亡[J]. 蚌埠医科大学学报, 2023, 48(11): 1510-1513. DOI: 10.13898/j.cnki.issn.1000-2200.2023.11.006
    引用本文: 刘旋, 张磊, 张凤军. lncRNA Zeb1-AS1调控JAK2/STAT3信号通路影响骨关节炎软骨细胞的增殖和凋亡[J]. 蚌埠医科大学学报, 2023, 48(11): 1510-1513. DOI: 10.13898/j.cnki.issn.1000-2200.2023.11.006
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    LIU Xuan, ZHANG Lei, ZHANG Feng-jun. lncRNA Zeb1-AS1 affects the proliferation and apoptosis of osteoarthritis chondrocytes via regulating JAK2/STAT3 signaling pathway[J]. Journal of Bengbu Medical University, 2023, 48(11): 1510-1513. DOI: 10.13898/j.cnki.issn.1000-2200.2023.11.006
    Citation: LIU Xuan, ZHANG Lei, ZHANG Feng-jun. lncRNA Zeb1-AS1 affects the proliferation and apoptosis of osteoarthritis chondrocytes via regulating JAK2/STAT3 signaling pathway[J]. Journal of Bengbu Medical University, 2023, 48(11): 1510-1513. DOI: 10.13898/j.cnki.issn.1000-2200.2023.11.006
    shu

    lncRNA Zeb1-AS1调控JAK2/STAT3信号通路影响骨关节炎软骨细胞的增殖和凋亡

    lncRNA Zeb1-AS1 affects the proliferation and apoptosis of osteoarthritis chondrocytes via regulating JAK2/STAT3 signaling pathway

      摘要
    • 摘要:
      目的探讨长链非编码RNA（lncRNA）E盒结合锌指蛋白1反义1（Zeb1-AS1）对骨关节炎软骨细胞的增殖和凋亡的影响，并探讨其机制是否与调控蛋白酪氨酸激酶2/信号转导和转录激活因子3（JAK2/STAT3）信号通路有关。
      方法将骨关节炎软骨细胞分为si-NC组、si-Zeb1-AS1组、si-Zeb1-AS1+JAK2/STAT3信号通路抑制剂（AG490）组。运用细胞计数试剂盒、流式细胞术检测细胞活力和凋亡率。Western blotting分析B细胞淋巴瘤（Bcl-2）、Bcl相关X蛋白（Bax）、p-JAK2和p-STAT3表达水平。
      结果与si-NC组比较，si-Zeb1-AS1组骨关节炎软骨细胞增殖（48、72 h）、Bcl-2、p-JAK2和p-STAT3蛋白表达明显升高（P < 0.01），凋亡率、Bax蛋白表达明显降低（P < 0.01）。与si-Zeb1-AS1组比较，si-Zeb1-AS1+AG490组骨关节炎软骨细胞增殖（48、72 h）、Bcl-2、p-JAK2和p-STAT3蛋白表达明显降低（P < 0.01），凋亡率、Bax蛋白表达明显升高（P < 0.01）。
      结论干扰lncRNA Zeb1-AS1通过激活JAK2/STAT3信号通路能够促进骨关节炎软骨细胞增殖、抑制细胞凋亡。

       

      Abstract:
      ObjectiveTo investigate the effect of long non-coding RNA (lncRNA) Zinc finger E-box binding homeobox 1 antisense 1 (Zeb1-AS1) on the proliferation and apoptosis of osteoarthritis chondrocytes and further explore whether its mechanism is related to the regulation of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway.
      MethodsThe osteoarthritis chondrocytes were divided into si-NC group, si-Zeb1-AS1 group, and si-Zeb1-AS1+JAK2/STAT3 signaling pathway inhibitor (AG490) group.Cell counting kit and flow cytometry were performed to detect cell viability and apoptosis rate.Western blotting was applied to analyze the expression levels of B cell lymphoma 2 (Bcl-2), Bcl-associated X protein (Bax), p-JAK2, and p-STAT3 proteins.
      ResultsCompared with the si-NC group, osteoarthritis chondrocyte viability (24 and 72 h), expressions of Bcl-2, p-JAK2, and p-STAT3 proteins were significantly increased, whereas apoptosis rate and Bax protein expression were significantly decreased in si-Zeb1-AS1 group (P < 0.01).Compared with the si-Zeb1-AS1 group, the osteoarthritis chondrocyte viability (24 and 72 h), and the expression of Bcl-2, p-JAK2 and p-STAT3 proteins were significantly reduced, whereas the apoptosis rate and Bax protein expression were significantly increased in si-Zeb1-AS1+AG490 group (P < 0.01).
      ConclusionsInterfering with lncRNA Zeb1-AS1 can promote the proliferation and inhibit apoptosis of osteoarthritis chondrocytes via activating the JAK2/STAT3 signaling pathway.

       

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