口腔鳞癌组织中LINC01605的表达及对细胞侵袭、迁移的影响

贾崴崴; 胡洋; 柳星光

doi:10.13898/j.cnki.issn.1000-2200.2023.12.003

口腔鳞癌组织中LINC01605的表达及对细胞侵袭、迁移的影响

Expression of LINC01605 in oral squamous cell carcinoma and its influences on cell invasion and migration

摘要

摘要:
目的探究LINC01605在口腔鳞癌组织中的表达及对细胞侵袭、迁移的影响。
方法收集口腔鳞癌病人的口腔鳞癌组织及相对应的癌旁组织标本各40例，分析口腔鳞癌组织中LINC01605的表达与临床病理特征的关系。将处于对数期的口腔鳞癌细胞CAL-27随机分为：CK组（正常培养）、si-NC组（转染si-NC）、si-LINC01605组（转染si-LINC01605）、pcDNA组（转染pcDNA）、pcDNA-LINC01605组（转染pcDNA-LINC01605）；qRT-PCR检测组织和细胞中LINC01605表达；CCK-8法检测细胞增殖；平板克隆实验检测细胞克隆形成能力；Transwell实验检测细胞侵袭；划痕实验检测细胞迁移；Western blotting检测细胞中增殖细胞核抗原（PCNA）、基质金属蛋白酶（MMP）-2、MMP-9、神经型钙黏素（N-cadherin）、上皮型钙黏蛋白（E-cadherin）表达；体内移植瘤实验检测肿瘤生长情况。
结果口腔鳞癌组织中LINC01605表达高于癌旁组织（P < 0.01）；LINC01605表达与TNM分期、分化程度、淋巴结转移有关（P < 0.05~P < 0.01）；与CK组、si-NC组比较，si-LINC01605组CAL-27细胞中吸光度值、克隆形成率、侵袭细胞数目、划痕愈合率、PCNA、MMP-2、MMP-9、N-cadherin蛋白表达及裸鼠体内移植瘤质量均降低（P < 0.01），E-cadherin蛋白表达升高（P < 0.01）；与CK组、pcDNA组比较，pcDNA-LINC01605组CAL-27细胞中对应指标变化趋势与上述相反（P < 0.01）。
结论LINC01605在口腔鳞癌组织中高表达，沉默LINC01605可抑制CAL-27细胞侵袭和迁移。

Abstract:
ObjectiveTo investigate the expression of LINC01605 in oral squamous cell carcinoma and its influences on cell invasion and migration.
MethodsForty oral squamous cell carcinoma tissues and corresponding paracancerous tissue specimens from patients with oral squamous cell carcinoma were collected, and the relationship between the expression of LINC01605 in oral squamous cell carcinoma tissues and clinicopathological characteristics was analyzed.Oral squamous cell carcinoma CAL-27 cells in logarithmic phase was randomly separated into: CK group (normal culture), si-NC group (transfected with si-NC), si-LINC01605 group (transfected with si-LINC01605), pcDNA group (transfected with pcDNA), and pcDNA-LINC01605 group (transfected with pcDNA-LINC01605).QRT-PCR was applied to detect LINC01605 expression in tissues and cells; CCK-8 assay was applied to detect cell proliferation; plate cloning assay was used to detect the ability of cells to form clones; Transwell assay was applied to detect cell invasion; scratch assay was applied to detect cell migration; Western blotting was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase (MMP)-2, MMP-9, neural cadherin (N-cadherin), and epithelial cadherin (E-cadherin) in cells; and in vivo xenograft assay was used to detect tumor growth.
ResultsThe expression of LINC01605 in oral squamous cell carcinoma tissue was greatly higher than that in adjacent tissue (P < 0.01).The expression of LINC01605 was related to TNM stage, degree of differentiation and lymph node metastasis (P < 0.05 to P < 0.01).Compared with CK group and si-NC group, the obsorbance value, clone formation rate, number of invasive cells, scratch healing rate, PCNA, MMP-2, MMP-9, N-cadherin protein expression, and the mass of transplanted tumor in CAL-27 cells in the si-LINC01605 group decreased (P < 0.01), while the expression of E-cadherin protein increased (P < 0.01).Compared with CK group and pcDNA group, the change trend of corresponding indexes in CAL-27 cells in pcDNA-LINC01605 group was opposite to the above (P < 0.01).
ConclusionsLINC01605 is highly expressed in oral squamous cell carcinoma tissues, and silencing LINC01605 can inhibit the invasion and migration of CAL-27 cells.

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