Abstract:
ObjectiveTo detect the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) on the surface of myeloid dendritic cells (mDC) and the expression levels of serum IL-10 and IL-17A in the peripheral blood of patients with systemic lupus erythematosus (SLE), and to investigate their role in the pathogenesis of SLE and their clinical significance.
MethodsA total of 54 patients with SLE were included as study subjects, and divided into stable and active groups according to the SLEDAI-2000, while 30 healthy volunteers were selected as the control group.Flow cytometry was applied to detect the expression of TLR2 and TLR4 on the surface of mDC in the peripheral blood of SLE patients and healthy volunteers, and ELISA was used to detect the expression of IL-10 and IL-17A in the serum of SLE patients and healthy volunteers.
ResultsThe expression rate of mDC-TLR2 in SLE patients in the active group was higher than that in the stable group and control group (P < 0.01).The expression rate of mDC-TLR4 was higher in the active group than that in the control group (P < 0.01).The expression levels of IL-10 and IL-17A in the serum of SLE patients were higher than those in the control group (P < 0.01).mDC-TLR2 expression was positively correlated with anti-Sm antibody (P < 0.05), and negatively correlated with serum IgG and 24h urine protein quantification (P < 0.05).The expression of mDC-TLR4 was positively correlated with platelet count (P < 0.05).
ConclusionsThe expression rates of mDC-TLR2 and TLR4 are increased in the active SLE group, and both may play a role in the pathogenesis or progression of SLE.
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