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    过敏性鼻炎病人外周血MALAT1、miR-17-5p的表达及临床意义

    Expression and clinical significance of MALAT1 and miR-17-5p in peripheral blood of patients with allergic rhinitis

      摘要
    • 摘要:
      目的 探讨过敏性鼻炎病人外周血肺腺癌转移相关转录本1(MALAT1)、微小RNA17-5p(miR-17-5p)的表达水平及其临床意义。
      方法 选取过敏性鼻炎病人125例作为观察组，择同期体检健康者62名作为对照组。以荧光定量聚合酶链反应检测受试者外周血MALAT1、miR-17-5p表达水平，绘制ROC曲线评估外周血MALAT1、miR-17-5p对过敏性鼻炎发生的诊断价值，Spearman相关性分析外周血MALAT1、miR-17-5p与病人疾病严重程度的相关性，再以多因素logistic回归分析影响过敏性鼻炎严重程度的独立危险因素。
      结果 观察组外周血MALAT1 mRNA水平低于对照组，miR-17-5p水平高于对照组(P < 0.05)；ROC曲线分析显示，MALAT1 mRNA曲线下面积为0.812，miR-17-5p曲线下面积为0.845，MALAT1 mRNA灵敏性优于miR-17-5p，特异性低于miR-17-5p，两者联合检测诊断价值更高；中重度过敏性鼻炎病人血清MALAT1 mRNA水平明显低于轻度病人(P < 0.05)，miR-17-5p水平明显高于轻度病人(P < 0.05)；Spearman相关性分析结果显示，外周血MALAT1 mRNA水平与过敏性鼻炎疾病严重程度呈负相关关系(r=-0.779，P < 0.01)，miR-17-5p水平与疾病严重程度呈正相关关系(r=0.774，P < 0.01)；多因素logistic回归分析结果显示，低水平MALAT1 mRNA、高水平miR-17-5p为影响过敏性鼻炎严重程度的独立危险因素(P < 0.05)。
      结论 过敏性鼻炎病人外周血中MALAT1 mRNA呈低表达，miR-17-5p呈高表达，与疾病严重程度关系密切，为影响过敏性鼻炎严重程度的独立危险因素，可能作为诊断过敏性鼻炎及评估疾病严重程度的潜在指标。

       

      Abstract:
      Objective To investigate the expression levels and clinical significance of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and miR-17-5p in peripheral blood of patients with allergic rhinitis.
      Methods A total of 125 patients with allergic rhinitis were selected as the observation group and 62 healthy subjects were selected as the control group.Fluorescence quantitative polymerase chain reaction was used to detect the expression levels of MALAT1 and miR-17-5p in peripheral blood of subjects, and ROC curve was applied to evaluate the diagnostic value of serum MALAT1 and miR-17-5p for the occurrence of allergic rhinitis.Spearman correlation was used to analyze the correlation between MALAT1 and miR-17-5p in peripheral blood and the clinical severity of allergic rhinitis, and multivariate logistic regression was used to analyze the independent risk factors affecting the severity of allergic rhinitis.
      Results The serum level of MALAT1 mRNA of observation group was lower than that of control group, and the serum level of miR-17-5p was higher than that of control group (P < 0.05).ROC curve analysis showed that the area under the curve of MALAT1 mRNA and miR-17-5p were 0.812 and 0.845 respectively.The sensitivity of MALAT1 mRNA was better than that of miR-17-5p, and the specificity was lower than that of miR-17-5p, when combined detection of MALAT1 mRNA and miR-17-5p resulted in a higher diagnostic value.The serum MALAT1 mRNA level in patients with moderate and severe allergic rhinitis was significantly lower than that in patients with mild allergic rhinitis (P < 0.05), and the serum miR-17-5p level was significantly higher than that in patients with mild allergic rhinitis (P < 0.05).Spearman correlation analysis showed that MALAT1 mRNA level in peripheral blood was negatively correlated with the severity of allergic rhinitis (r=-0.779, P < 0.01), and miR-17-5p level was positively correlated with the severity of allergic rhinitis (r=0.774, P < 0.01).Multivariate logistic regression analysis showed that low MALAT1 mRNA level and high miR-17-5p level were independent risk factors for the severity of allergic rhinitis (P < 0.05).
      Conclusions The expression of MALAT1 mRNA in peripheral blood of patients with allergic rhinitis is low, and miR-17-5p is high.They are closely related to the severity of allergic rhinitis, which are independent risk factors pertaining to its clinical severity.Serum level of MALAT1 mRNA and miR-17-5p may be of diagnostic and severity assessment value in patients with allergic rhinitis.

       

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