急性髓系白血病病人免疫球蛋白κ链C区蛋白和趋化因子配体2表达及意义

黄丹; 秦超; 陈书成

doi:10.13898/j.cnki.issn.1000-2200.2024.05.019

急性髓系白血病病人免疫球蛋白κ链C区蛋白和趋化因子配体2表达及意义

Expression levels and significance of chemokine C-C motif ligand 2 and protein of C region of immunoglobulin κ chain in patients with acute myeloid leukemia

摘要

摘要:
目的探讨急性髓系白血病病人免疫球蛋白κ链C区蛋白(IGKC)、趋化因子配体2(CCL2)表达水平及临床意义。
方法选取急性髓系白血病病人80例作为观察组，另选取同期体检健康者40名作为对照组。采用酶联免疫吸附法检测2组病人血清IGKC、CCL2水平，并比较观察组不同分型病人血清IGKC、CCL2水平；Pearson法分析观察组病人IGKC、CCL2水平与降钙素原(PCT)、C反应蛋白(CRP)的相关性；ROC曲线分析血清IGKC、CCL2、PCT及CRP水平对观察组不同分型病人的诊断价值。
结果观察组病人血清IGKC、CCL2、PCT、CRP水平均明显高于对照组(P＜0.01)；不同WHO分型、ELN分型病人血清IGKC、CCL2、PCT、CRP水平间差异均有统计学意义(P＜0.01)，其中，M3~M4型、M5~M6型病人血清IGKC、CCL2、PCT、CRP水平均高于M1~M2型(P＜0.05)；与良好型病人相比，中等型和不良型病人血清IGKC、CCL2、PCT、CRP水平均高于良好型病人(P＜0.05)。Pearson相关分析显示，观察组病人血清IGKC与CCL2、PCT、CRP均呈正相关关系(r=0.393、0.302、0.487，P＜0.05)，CCL2与PCT、CRP均呈正相关关系(r=0.510、0.516，P＜0.05)。ROC曲线分析结果显示，血清IGKC、CCL2诊断M1~M2型及M5~M6型急性髓系白血病水平较高，AUC均达到0.75以上。
结论急性髓系白血病病人血清IGKC、CCL2均呈高表达，二者对急性髓系白血病有一定的辅助诊断价值。

Abstract:
Objective To investigate the expression levels and clinical significance of protein of C region of immunoglobulin κ chain (IGKC) and chemokine C-C motif ligand 2(CCL2) in patients with acute myeloid leukemia.
Methods Eighty patients with acute myeloid leukemia and 40 healthy people during the same period were divided into the observation group and control group. The serum levels of IGKC and CCL2 in two groups were detected by enzyme-linked immunosorbent assay, and the serum levels of IGKC and CCL2 of different types of patients in observation group were compared. The correlations between the levels of IGKC and CCL2 and levels of procalcitonin(PCT) and C-reactive protein(CRP) in the observation group were analyzed by Pearson method. The diagnostic value of serum levels of IGKC, CCL2, PCT and CRP of different types of patients in the observation group were analyzed using ROC curve.
Results The serum levels of IGKC, CCL2, PCT and CRP in the observation group were significantly higher than those in the control group (P < 0.01). The differences of the serum levels of IGKC, CCL2, PCT and CRP in patients with different WHO and ELN type were statistically significant (P < 0.01), and the serum levels of IGKC, CCL2, PCT and CRP in patients with M3-M4 and M5-M6 types were higher than those in patients with M1-M2 type (P < 0.05). The serum levels of IGKC, CCL2, PCT and CRP in moderate and bad type patients were higher than those in good type patients (P < 0.05). The results of Pearson correlation analysis showed that the serum level of IGKC was positively correlated with CCL2, PCT and CRP in the observation group (r=0.393, 0.302, 0.487, P < 0.05), and the serum level of CCL2 was positively correlated with PCT and CRP (r=0.510, 0.516, P < 0.05). The results of ROC curve analysis showed that the serum levels of IGKC and CCL2 had higher diagnostic value in the M1-M2 and M5-M6 type acute myeloid leukemia, and the AUC was above 0.75.
Conclusions The serum levels of IGKC and CCL2 are high in the patients with acute myeloid leukemia, and both have a certain auxiliary diagnosis value in acute myeloid leukemia.

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