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    99Tcm-DTPA-CGRRAGGSC在非小细胞肺癌裸鼠模型的显像研究

    Imaging study on the 99Tcm-DTPA-CGRRAGGSC scintigraphy in the nude mice model with non-small cell lung cancer

      摘要
    • 摘要:
      目的 探讨放射性核素99Tcm标记环九肽(CGRRAGGSC)对荷非小细胞肺癌裸鼠的显像研究。
      方法 用99Tcm标记DTPA-CGRRAGGSC，测定其标记率及稳定性，制备荷非小细胞肺癌小鼠模型，经荷瘤鼠尾静脉注射标记物2 h后进行SPECT静态显像，并计算出靶与非靶(T/NT)比值。
      结果 成功制备出99Tcm-DTPA-CGRRAGGSC，在PBS和人血清中37 ℃放置6 h时放射性化学纯度在90%以上；经荷瘤鼠尾静脉注射标记物2 h后T/NT比值最高达3.5。
      结论 99Tcm标记DTPA-CGRRAGGSC制备简便，其对荷非小细胞肺癌裸鼠具有较高靶向性，99Tcm-DTPA-CGRRAGGSC有可能在IL-11Rα高表达的非小细胞肺癌分子显像中发挥重要作用。

       

      Abstract:
      Objective To explore the imaging of scintigraphy of CGRRAGGSC labeled with 99Tcm in nude mice with non-small cell lung cancer.
      Methods The DTPA-CGRRAGGSC was labeled with 99Tcm, and the labeling rate and stability were measured.The mice model with non-small cell lung cancer were established.The markers were injected into the tail vein of the tumor-bearing mice, the SPECT static imaging was performed after 2 hours, and the ratio of target to non-target(T/NT) was calculated.
      Results The 99Tcm-DTPA-CGRRAGGSC was successfully radiolabeled, and the radiochemical purity of which was above 90% when it was placed in PBS and human serum at 37 ℃ for 6 h.The T/NT ratio was as high as 3.5 after 2 hours of injection of the marker through the tail vein of tumor-bearing mice.
      Conclusions The 99Tcm-labeled DTPA-CGRRAGGSC is easy to be prepared, and it has high targeting to nude mice with non-small cell cancer.99Tcm-DTPA-CGRRAGGSC may play an important role in molecular imaging of non-small cell lung cancer with high expression of IL-11Rα.

       

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