Abstract: Objective: To investigate the influence of different rehabilitation timing on acute ischemic stroke patients in early rehabilitation. Methods: A total of 80 patients with acute ischemic stroke were randomly divided into the 24 h group and 72 h group(40 cases in each group).The rehabilitation exercise program and mode of the two groups were the same,and the rehabilitation exercise was carried out at 24 h and 72 h after stroke,respectively.The differences of neurological function(NIHSS),motor function(FMA) and ability of daily living(BI) before and after rehabilitation exercise were compared between two groups. Results: Before rehabilitation exercise,there was no statistical significance in the NIHSS,FMA and BI scores between two groups(P>0.05).After rehabilitation and 3 months of rehabilitation,the NIHSS score in two groups significantly decreased(P<0.01),and the decreasing degree of 24 h group was higher than that of 72 h group(P<0.01).The motion and BI scores of upper and lower limbs in two groups were significantly increased(P<0.01),and the increasing degree in the 24 h group was higher than that in the 72 h group(P<0.01). Conclusions: For patients with mild to moderate acute ischemic stroke,the effect of rehabilitation exercise based on muscle strength assessment at 24 h after stroke is better than that at 72 h.