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    冠心病病人外周血单个核细胞中USP22、miR-520b表达与冠状动脉CTA钙化积分的相关性分析

    Correlation analysis of USP22 and miR-520b expression in peripheral blood mononuclear cells and coronary CTA calcium score of patients with coronary heart disease

      摘要
    • 摘要:
      目的 探讨冠心病(CHD)病人外周血单个核细胞(PBMC)中泛素特异性蛋白酶22(USP22)、微小RNA(miR)-520b表达与冠状动脉CTA钙化积分(CACS)的相关性。
      方法 以110例CHD病人为CHD组, 同期健康志愿者为对照组, 采集外周血测定PBMC中USP22、miR-520b表达水平。CHD组根据CACS分级标准分为无冠状动脉钙化(CAC)组(20例)、少CAC组(16例)、轻CAC组(23例)、中CAC组(29例)、重CAC组(22例)。比较各组USP22、miR-520b表达水平差异, 分析CHD病人发生CAC的影响因素。Pearson系数分析USP22、miR-520b表达水平与CACS的相关性。
      结果 CHD组USP22表达水平(1.87±0.28)显著高于对照组(1.12±0.16)(P < 0.01), miR-520b表达水平(0.64±0.12)低于对照组(1.06±0.15)(P < 0.01)。CHD组不同钙化程度分组间USP22表达水平符合无CAC组 < 少CAC组 < 轻CAC组 < 中CAC组 < 重CAC组(P < 0.01), miR-520b表达水平符合无CAC组>少CAC组>轻CAC组>中CAC组>重CAC组(P < 0.01)。CHD组PBMC中USP22表达水平与CACS呈正相关关系(r=0.560, P < 0.01), miR-520b表达水平与CACS呈负相关关系(r=-0.593, P < 0.01)。随着冠状动脉病变支数增加, USP22表达水平升高, miR-520b表达水平降低(P < 0.01)。PBMC中USP22、miR-520b是影响CHD病人发生CAC的相关因素(P < 0.01和P < 0.05)。
      结论 CHD病人PBMC中USP22表达水平升高, miR-520b表达水平降低, 且与CAC存在显著相关性, 并对临床预测CHD病人CAC有一定参考价值。

       

      Abstract:
      Objective To investigate the correlation between the expression of ubiquitin-specific protease 22 (USP22) and microRNA (miR)-520b in peripheral blood mononuclear cells (PBMC) and coronary CTA calcium score (CACS) of patients with coronary heart disease (CHD).
      Methods A total of 110 CHD patients were set as the CHD group, and healthy volunteers in the same period were set as the control group.The peripheral blood was collected to determine the expression levels of USP22 and miR-520b in PBMC.The CHD group was grouped into no coronary artery calcification (CAC) group (20 cases), less CAC group (16 cases), mild CAC group (23 cases), moderate CAC group (29 cases), and severe CAC group (22 cases) according to the CACS grading standard.The differences in the expression levels of USP22 and miR-520b in each group were compared, and the influencing factors of CAC in CHD patients were analyzed.Pearson coefficient was performed to analyze the correlation of the expression levels of USP22 and miR-520b with CACS.
      Results The expression level of USP22 in the CHD group (1.87±0.28) was notably higher than that in the control group (1.12±0.16) (P < 0.01), and the expression level of miR-520b (0.64±0.12) was lower than that in the control group (1.06±0.15) (P < 0.01).Among the groups of different calcification degrees in CHD groupthe expression level of USP22 was consistent with no CAC group < less CAC group < mild CAC group < moderate CAC group < heavy CAC group (P < 0.01), and the expression level of miR-520b was consistent with no CAC group >less CAC group>mild CAC group>moderate CAC group >heavy CAC group (P < 0.01).The expression level of USP22 in PBMC of CHD group was positively correlated with CACS (r=0.560, P < 0.01), and the expression level of miR-520b was negatively correlated with CACS (r=-0.593, P < 0.01).With the increase in the number of coronary lesions, the expression level of USP22 increased and the expression level of miR-520b decreased (P < 0.01).USP22 and miR-520b in PBMC were related factors affecting the occurrence of CAC in CHD patients (P < 0.01 and P < 0.05).
      Conclusions The expression level of USP22 in PBMC of CHD patients is increased, and the expression level of miR-520b is decreased, which is notably correlated with CAC, and has a certain reference value for clinical prediction of CAC in CHD patients.

       

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