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    慢性牙周炎病人唾液中lncRNA FGD5-AS1表达水平与疾病严重程度的相关性分析

    Correlation analysis of lncRNA FGD5-AS1 expression level in saliva and disease severity of patients with chronic periodontitis

      摘要
    • 摘要:
      目的 探讨慢性牙周炎(CP)病人唾液中长链非编码RNA含有5个PH结构域的反义RNA1(lncRNA FGD5-AS1)与疾病严重程度的关系。
      方法 选取138例CP病人作为观察组, 根据病人病情严重程度分为轻度CP组39例, 中度CP组51例, 重度CP组48例。另选取同时期体检的健康者59例作为对照组；采用实时荧光定量PCR(qRT-PCR)法测定所有受试者唾液中lncRNA FGD5-AS1、miR-142-3p相对表达量；采用Pearson法分析lncRNA FGD5-AS1、miR-142-3p与菌斑指数(PLI)、牙龈指数(GI)、牙周袋探诊深度(PD)、临床附着丧失(CAL)、出血指数(BI)的相关性及lncRNA FGD5-AS1、miR-142-3p水平之间的相关性；使用ROC曲线分析唾液lncRNA FGD5-AS1、miR-142-3p对CP的诊断价值；多因素logistic回归分析影响CP的危险因素。
      结果 观察组病人唾液lncRNA FGD5-AS1水平明显低于对照组(P < 0.01), miR-142-3p水平明显高于对照组(P < 0.01)；CP越严重, 病人lncRNA FGD5-AS1水平越低(P < 0.01), miR-142-3p水平越高(P < 0.01)；观察组病人lncRNA FGD5-AS1水平与PLI、GI、PD、CAL、BI、miR-142-3p呈负相关(P < 0.01), miR-142-3p水平与PLI、GI、PD、CAL、BI呈正相关(P < 0.05~P < 0.01)；ROC曲线分析显示, 与lncRNA FGD5-AS1单独诊断相比, 二者联合诊断CP的AUC更高(P < 0.05), 与miR-142-3p单独诊断相比, 二者联合诊断CP的AUC差异无统计学意义(P>0.05)；多因素logistic回归分析表明, lncRNA FGD5-AS1低水平和miR-142-3p高水平是CP发生的独立危险因素(P < 0.05和P < 0.01)。
      结论 CP病人唾液中lncRNA FGD5-AS1表达水平降低, miR-142-3p表达水平升高, 二者与病人病情严重程度密切相关。

       

      Abstract:
      Objective To investigate the relationship between long non-coding RNA PH domain containing 5 antisense RNA1 (lncRNA FGD5-AS1) in saliva of patients with chronic periodontitis (CP) and disease severity.
      Methods A total of 138 CP patients were selected as the observation group.According to the severity of the patients, they were divided into mild CP group with 39 cases, moderate CP group with 51 cases, and severe CP group with 48 cases.In addition, 59 healthy people for physical examination were selected as the control group.Real-time fluorescence quantitative PCR (qRT-PCR) was performed to determine the relative expression levels of lncRNA FGD5-AS1 and miR-142-3p in the saliva of subjects.Pearson method was performed to analyze the correlation between lncRNA FGD5-AS1, miR-142-3p and plaque index (PLI), gingival index (GI), probing depth of periodontal pocket (PD), clinical attachment loss (CAL), bleeding index (BI), and the correlation between the levels of lncRNA FGD5-AS1 and miR-142-3p.ROC curve was performed to analyze the diagnostic value of salivary lncRNA FGD5-AS1 and miR-142-3p for CP.Multivariate logistic regression was performed to analyze the risk factors for CP.
      Results The salivary lncRNA FGD5-AS1 level of the observation group was obviously lower than that of the control group, and the miR-142-3p level was obviously higher than that of the control group (P < 0.01).The more severe the CP, the lower the lncRNA FGD5-AS1 level in patients (P < 0.01), and the higher the miR-142-3p level (P < 0.01).The level of lncRNA FGD5-AS1 in the observation group was negtively correlated with PLI, GI, PD, CAL, BI, and miR-142-3p (P < 0.01), and the level of miR-142-3p was positively correlated with PLI, GI, PD, CAL, and BI (P < 0.05 to P < 0.01).ROC curve analysis showed that compared with the single diagnosis of lncRNA FGD5-AS1, the AUC of the two combined diagnosis of CP was higher (P < 0.05), and compared with the single diagnosis of miR-142-3p, the AUC of the two combined diagnosis of CP was not obviously different (P>0.05).Multivariate logistic regression analysis showed that low level of lncRNA FGD5-AS1 and high level of miR-142-3p were independent risk factors for the occurrence of CP (P < 0.05 and P < 0.01).
      Conclusions The expression level of lncRNA FGD5-AS1 in the saliva of CP patients is decreased, and the expression level of miR-142-3p is increased, and the two are tightly related to the severity of the patient's disease.

       

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