高级检索

    USP10和CLIC3在喉鳞状细胞癌组织中的表达及意义

    Expression and significance of USP10 and CLIC3 in laryngeal squamous cell carcinoma

      摘要
    • 摘要:
      目的 探讨泛素特异性蛋白酶10(USP10)和细胞内氯离子通道蛋白3(CLIC3)在喉鳞状细胞癌中的表达及临床意义；以及在喉鳞状细胞癌中USP10和CLIC3蛋白表达的相关性。探讨USP10与CLIC3信号通路是否相同。
      方法 收集经手术病理证实的60例喉鳞状细胞癌病人的癌组织及癌旁正常黏膜组织(距肿瘤切缘>5 mm)标本, 应用免疫组织化学法检测组织标本中USP10、CLIC3的表达水平, 并进行统计学分析, 探讨两者的相关性。
      结果 USP10蛋白在细胞核和细胞质中表达, 喉癌组织中USP10的阳性率为81.67%, 高于癌旁正常组织表达率的21.67%(P < 0.01)；CLIC3蛋白在细胞核中表达, 喉癌组织中CLIC3的阳性表达率为33.33%, 显著低于癌旁组织表达率68.33%(P < 0.01)；临床分期高、分化程度低和有淋巴结转移者的LSCC中USP10阳性表达率分别高于临床分期低、分化程度高和无淋巴结转移者的阳性率(P < 0.05~P < 0.01)。
      结论 USP10在喉癌中起到促癌基因的作用, CLIC3在喉癌中起到抑癌基因的作用, 通过检测USP10和CLIC3可对喉鳞状细胞癌的发展及预后评估提供一定的理论依据。

       

      Abstract:
      Objective : To explore the expression and clinical significance of ubiquitin specific protease 10 (USP10) and the chloride intracellular channel protein 3 (CLIC3) in laryngeal squamous cell carcinoma, and to explore the correlation of the expression between USP10 and CLIC3.
      Methods : The specimens of 60 cancer tissues and adjacent normal mucosa tissues (more than 5 mm from the tumor resection margin) of the laryngeal cancer patients were collected.The expression levels of USP10 and CLIC3 in tissue specimens were determined by immunohistochemistry and statistical analysis was conducted to explore the correlation between USP10 and CLIC3.
      Results The USP10 protein was expressed both in the nucleus and in the cytoplasm.The expression rate of USP10 in laryngeal carcinoma tissues was 81.67%, which was higher than 21.67% of normal adjacent tissues (P < 0.05).The CLIC3 protein was expressed in the nucleus.The expression rate of CLIC3 in laryngeal carcinoma tissues was 33.33%, which was significantly lower than that in adjacent tissues (P < 0.01).The positive rate of USP10 expression in LSCC with high clinical stage, low differentiation and with lymph node metastasis was higher than that of those with low clinical stage, high differentiation and without lymph node metastasis, respectively (P < 0.05 to P < 0.01).
      Conclusions USP10 acts as a pro-oncogene in laryngeal squamous cell carcinoma.CLIC3 acts as a tumor suppressor gene in laryngeal squamous cell carcinoma.Testing of USP10 and CLIC3 can provide some theoretical basis for the development and prognosis evaluation of laryngeal squamous cell carcinoma.

       

      • HTML全文
      • 参考文献(28)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回