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    甲状腺结节中细胞凋亡与FAS、FASL的表达水平及临床意义

    Study on the apoptosis and expression levels of FAS and FASL in thyroid nodules and their clinical significance

      摘要
    • 摘要:
      目的： 探讨甲状腺细胞（TEC）凋亡率及脂肪酸合成酶（FAS）、脂肪酸合成酶配体（FASL）表达在甲状腺结节中的意义。
      方法： 选取甲状腺结节病人75例，收集术中切除的甲状腺病变组织，按病理学诊断结果，将标本分为结节性甲状腺肿组（腺肿组，n = 27）、甲状腺腺瘤组（腺瘤组，n = 21）和甲状腺癌组（n = 27），另选取甲状腺癌旁正常组织（n = 27）作为对照组。采用TUNEL法检测4组TEC凋亡率；免疫组织化学法检测FAS和FASL表达水平。
      结果： 腺肿组、腺瘤组和甲状腺癌组TEC凋亡率均高于对照组（P ＜ 0.05），FAS、FASL表达水平亦均高于对照组（P ＜ 0.05）。甲状腺组织中TEC凋亡率与FAS、FASL阳性表达率均呈明显正相关关系(r = 0.940、0.943，P ＜ 0.01）。
      结论： TEC的异常凋亡可能参与甲状腺结节的病变过程，FAS和FASL可能是导致TEC异常凋亡的重要原因，并且可能与甲状腺结节的病理分型有关。

       

      Abstract:
      Objective To investigate the apoptosis rate of thyroid cells (TEC) and the expression significance of FAS, FASL in thyroid nodules.
      Method Seventy-five patients with thyroid nodules were selected to collect the thyroid lesion tissues. According to the pathological diagnosis results, the specimens were divided into the nodular goiter group (goiter group, n = 27), thyroid adenoma group (adenoma group, n = 21) and thyroid cancer group (n = 27), and the normal tissue adjacent to thyroid cancer was selected as the control group. TUNEL method was used to detect the apoptosis rate of TEC in 4 groups. The expression levels of FAS and FASL were detected by immunohistochemistry.
      Results The apoptosis rate of TEC in the goiter group, adenoma group and thyroid cancer group was higher than that in control group (P ＜ 0.05), and the expression levels of FAS and FASL were also higher than those in control group (P ＜ 0.05). The apoptosis rate of TEC was positively correlated with the positive expression rates of FAS and FASL in thyroid tissues (r = 0.940, 0.943, P ＜ 0.01).
      Conclusions The abnormal apoptosis of TEC may be involved in the pathological process of thyroid nodules. FAS and FASL may be important causes of the abnormal apoptosis of TEC, and may be related to the pathological types of thyroid nodules.

       

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