Abstract: Objective: To study the effect of long non-coding ribonucleic acid taurine up-regulated gene 1(lncRNA TUG1) on dentin differentiation of human dental pulp stem cells(hDPSCs) by regulating the wingless-type MMTV integration site family/β-catenin (Wnt/β-catenin) pathway. Methods: HDPSCs were isolated and cultured in vitro,and divided into the blank control group,negative control group(siRNA-NC group),silent TUG1 expression group (TUG1-siRNA group) and combined group(TUG1-siRNA+ activation agent of Wnt/β-catenin pathway lithium chloride).The real-time fluorescence quantitative PCR was used to detect the expression of TUG1;the proliferation ability of hDPSCs was detected by CCK-8;alizarin red staining method was used to to detect the differentiation effect of dentin,and the expression levels of dentin differentiation-related proteinshuman neuropilin 1 (NRP1),dentin matrix protein(DMP1),dentin sialoprotei(DSPP),Wnt and β-catenin proteins were detected by Western blotting. Results: Compared with the blank control group and siRNA-NC group,the proliferation inhibition rate of hDPSCs significantly increased,and the dark red calcified nodules forming ability and expression levels of NRP1,DSPP,DMP1,Wnt,and β-catenin protein significantly reduced in the TUG1-siRNA group(P<0.05).Compared with the TUG1-siRNA group,the proliferation inhibition rate of hDPSCs significantly reduced,and the dark red calcified nodules forming ability and expression levels of NRP1,DSPP,DMP1,Wnt and β-catenin proteins significantly increased in the combined group(P<0.05). Conclusions: Silencing lncRNA TUG1 may inhibit the proliferation of hDPSCs and dentin differentiation by inhibiting the activation of Wnt/β-catenin signaling pathway.