Abstract: Objective: To investigate the clinical application of No-touch technique in the establishment of wrist standard arteriovenous fistula(SAVF) by cephalic vein separation. Methods: The clinical data of 95 patients undergoing left or right forearm SAVF surgery to maintain long-term dialysis were analyzed retrospectively.According to the method of vein separation,50 cases were set as No-touch technique group(group N) and 45 cases were set as traditional technique group(group T).The operation success rate,clinical success rate and complications were compared between the two groups during the follow-up period. Results: During the follow-up period,the operation success rate in the two groups was 100.0%.The number of forearm edema cases of short-term complications in group T was significantly higher than that in group N(P<0.01),and there was no significant difference in the number of bleeding,pain and vasospasm cases between the two groups(P>0.05).Kaplan-Meier survival analysis showed that in group N was 84.0%(42/50),which was significantly higher than 60.0%(27/45) in group T at the end of the follow-up period(P<0.01),and Log-Rank test showed that the clinical sows nate in the clinical success rate in group N was better than group T(P<0.05).The incidence of type Ⅰ stenosis and dysplasia of internal fistula in group N was lower than that in group T(P<0.05),and there was no significant difference in the number of other types of stenosis,dysplasia and other cause-induced occlusion between the two groups(P>0.05). Conclusions: It can significantly reduce the incidence of type Ⅰ stenosis and improve the clinical success rate of operation to apply No-touch technique to separate cephalic vein for SAVF establishment,which is worthy of clinical application.