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    栀子苷调控IKK/NF-κB通路对大鼠牙周炎症及牙龈血管生成的影响

    Effect of geniposide on periodontal inflammation and gingival angiogenesis in rats by regulating the IKK/NF-κB pathway

      摘要
    • 摘要:
      目的 探讨栀子苷对牙周炎症和牙龈血管生成的影响，并基于κB抑制因子激酶(IKK)/核因子(NF)-κB信号通路初步探讨其作用机制。
      方法 采用结扎法建立实验性牙周炎大鼠模型，建模成功大鼠40只，随机分为模型组和低、中、高剂量栀子苷组，各10只，低、中、高剂量栀子苷组大鼠分别给予5、10、20 mg/kg栀子苷灌胃；另选取大鼠10只作为对照组，不作建模处理。采用Micro-CT成像评估各组大鼠牙槽骨吸收量、骨矿密度(BMD)、骨体积/组织体积(BV/TV)；采用蛋白免疫印迹法、免疫组织化学法分析各组组织病理损伤和相关蛋白表达。
      结果 与对照组比较，模型组BMD、BV/TV均降低，而牙槽骨吸收量、牙龈组织中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、PECAM、血管内皮生长因子(VEGF)、p-IKKα、p-IKKβ、p-IκB、p-NF-κB p65蛋白表达均升高(P < 0.05)；与模型组比较，低、中、高剂量栀子苷组BMD、BV/TV均升高，牙槽骨吸收量和牙龈组织中TNF-α、IL-1β、PECAM、VEGF、p-IKKα、p-IKKβ、p-IκB、p-NF-κB p65蛋白表达均降低(P < 0.05)。
      结论 栀子苷有助于修复牙周炎大鼠牙槽骨丢失，抑制牙周炎症反应，其机制可能与IKK/NF-κB信号通路抑制有关。

       

      Abstract:
      Objective To investigate the effects of geniposide on periodontal inflammation and gingival angiogenesis, and to preliminarily explore its mechanism of action based on the IKK/NF-κB signaling pathway.
      Methods A rat model of experimental periodontitis was established using the ligation method.Forty successfully modeled rats were randomly divided into a model group and low, medium, high geniposide groups, with 10 rats in each group.The rats in the low, medium, high geniposide groups were administered 5, 10, 20 mg/kg geniposide via gavage, respectively.An additional 10 rats were selected as the control group and did not undergo modeling.Micro-CT imaging was used to evaluate alveolar bone resorption, bone mineral density (BMD), and bone volume/tissue volume (BV/TV) in each group.Western blotting and immunohistochemistry were employed to analyze histopathological damage and expression of related proteins in each group.
      Results Compared with the control group, the model group exhibited decreased BMD and BV/TV, while the alveolar bone resorption and protein expression of TNF-α, IL-1β, PECAM, VEGF, p-IKKα, p-IKKβ, p-IκB, and p-NF-κB p65 in gingival tissues were significantly increased (P < 0.05).Compared with the model group, the low, medium, and high dose geniposide groups showed increased BMD and BV/TV, along with reduced alveolar bone resorption and decreased expression of TNF-α, IL-1β, PECAM, VEGF, p-IKKα, p-IKKβ, p-IκB, and p-NF-κB p65 in gingival tissues (P < 0.05).
      Conclusions Geniposide facilitates the repair of alveolar bone loss and inhibits the periodontal inflammatory response in rats with periodontitis, the mechanism of which may be associated with the suppression of IKK/NF-κB signaling pathway.

       

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