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    外周血组蛋白脱乙酰酶4的表达与绝经后骨质疏松症病人骨密度相关指标的关系

    Relationship between histone deacetylase 4 expression in peripheral blood and bone mineral density-related indicators in postmenopausal osteoporosis patients

      摘要
    • 摘要:
      目的 探讨外周血组蛋白脱乙酰基酶4(HDAC4)表达水平与绝经后骨质疏松症(PMO)病人骨密度(BMD)相关指标的关系。
      方法 选取女性PMO病人82例作为观察组，同期女性健康体检者82名作为对照组。实时荧光定量PCR检测血清HDAC4 mRNA水平；使用双能X线骨密度仪检测受试者BMD；Pearson法分析PMO病人血清HDAC4 mRNA表达水平与Ⅰ型胶原氨基端延长肽(P1NP)、β-Ⅰ型胶原羧基端肽(β-CTX)、腰椎BMD、股骨颈BMD的相关性；logistic回归分析影响PMO发生的因素；绘制ROC曲线评估血清HDAC4 mRNA表达水平对PMO的预测价值。
      结果 与对照组相比，观察组病人血清PINP、腰椎BMD、股骨颈BMD水平明显降低(P < 0.01)，HDAC4 mRNA、β-CTX水平明显升高(P < 0.01)。Pearson相关分析显示，PMO病人血清HDAC4 mRNA表达水平与PINP水平、腰椎BMD、股骨颈BMD均呈明显负相关关系(r=-0.399、-0.437、-0.492，P < 0.01)，与β-CTX水平呈明显正相关关系(r=0.456，P < 0.01)。logistic回归分析显示，血清β-CTX、HDAC4 mRNA水平升高为PMO发生的独立危险因素(P < 0.01)。ROC曲线分析显示，血清HDAC4 mRNA预测PMO的AUC为0.878(95%CI: 0.823~0.932)，灵敏度为80.5%，特异度为94.1%。
      结论 PMO病人血清HDAC4 mRNA表达水平显著升高，与PINP水平、BMD呈明显负相关关系，与β-CTX水平呈明显正相关关系，血清β-CTX、HDAC4 mRNA水平升高是PMO发生的独立危险因素。

       

      Abstract:
      Objective To investigate the relationship between peripheral blood histone deacetylase 4 (HDAC4) expression in peripheral blood and bone mineral density (BMD)-related indicators in postmenopausal osteoporosis (PMO) patient.
      Methods Eighty-two female PMO patients were selected as the observation group, and 82 female health examinees during the same period were selected as the control group. Real time fluorescence quantitative PCR was used to detect the serum HDAC4 mRNA levels; dual energy X-ray absorptiometry was employed to measure the BMD of the subjects; Pearson method was applied to analyze the correlation between serum HDAC4 mRNA expression levels and procollagen type Ⅰ aminoterminal propeptide (PINP), cross-linked C-terminal telopeptide of type Ⅰ collagen (β-CTX), lumbar spine BMD, and femoral neck BMD in PMO patients; logistic regression analysis was used to analyze the influencing factors for PMO occurrence; ROC curve was drawn to evaluate the predictive value of serum HDAC4 mRNA expression level for PMO.
      Results Compared with the control group, the levels of serum PINP, lumbar spine BMD, and femoral neck BMD in the observation group were significantly reduced (P < 0.01), while the levels of HDAC4 mRNA and β-CTX were significantly increased (P < 0.01). Pearson correlation analysis showed that the expression level of serum HDAC4 mRNA in PMO patients was significantly negatively correlated with PINP level, lumbar spine BMD, and femoral neck BMD (r=-0.399, -0.437, -0.492, P < 0.01), and significantly positively correlated with β-CTX level (r=0.456, P < 0.01). Logistic regression analysis showed that the elevated levels of serum β-TX and HDAC4 mRNA were independent risk factors for PMO (P < 0.01). ROC curve analysis showed that the AUC of serum HDAC4 mRNA to predict PMO was 0.878 (95%CI: 0.823-0.932), the sensitivity was 80.5%, and the specificity was 94.1%.
      Conclusions The expression level of serum HDAC4 mRNA in PMO patients is significantly up-regulated, which is negatively correlated with PINP level and BMD, and positively correlated with β-CTX level. The increased levels of serum β-CTX and HDAC4 mRNA expression are independent risk factors for PMO.

       

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