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    白桦脂酸对人鼻咽癌HNE-1细胞增殖、迁移的作用研究

    Study on the effects of betulinic acid on the proliferation and migration of human nasopharyngeal carcinoma HNE-1 cells

      摘要
    • 摘要:
      目的 探讨白桦脂酸(BA)对鼻咽癌(NPC)细胞HNE-1细胞生物学功能的影响和作用机制。
      方法 使用CCK8法检测不同浓度的BA对HNE-1细胞活力的影响；通过克隆形成实验检测BA对细胞增殖能力的影响；使用划痕愈合实验、Transwell迁移实验方法检测对细胞迁移能力的影响；转录组测序分析BA影响HNE-1细胞的信号通路，并通过蛋白免疫印记实验验证通路相关蛋白的表达情况。
      结果 BA可抑制HNE-1细胞的增殖、克隆形成和迁移能力，且随着浓度的升高，BA抑制HNE-1细胞的增殖、克隆形成和迁移能力增强(P＜0.01)，其机制可能是通过影响MAPK信号通路。
      结论 BA可通过抑制MAPK信号通路减弱HNE-1细胞的增殖和迁移能力。

       

      Abstract:
      Objective To explore the effects of betulinic acid (BA) on the biological functions of nasopharyngeal carcinoma (NPC) HNE-1 cells, and its mechanism of action.
      Methods The effect of different concentrations of BA on the viability of HNE-1 cells was detected by CCK8 method. The effect of BA on cell proliferation ability was detected by clone formation assay. The effects on the migration ability of cells were detected by scratch healing assay and Transwell migration assay. Transcriptome sequencing was used to analyze the signaling pathways of HNE-1 cells affected by BA, and the expression of pathway-related proteins was verified by Western blotting.
      Results BA inhibited the proliferation, clone formation and migration ability of HNE-1 cells. Moreover, with the increase of concentration, the inhibitory ability of BA on the proliferation, clone formation and migration of HNE-1 cells increased (P < 0.01), and the mechanism might be by affecting the MAPK signaling pathway.
      Conclusions BA can weaken the proliferation and migration ability of HNE-1 cells by inhibiting the MAPK signaling pathway.

       

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