鲁正, 朱言亮, 何长林, 刘牧林, 刘金新. 脾脏在大鼠急性胰腺炎炎症介质释放中的作用[J]. 蚌埠医学院学报, 2005, 30(2): 110-112.
    引用本文: 鲁正, 朱言亮, 何长林, 刘牧林, 刘金新. 脾脏在大鼠急性胰腺炎炎症介质释放中的作用[J]. 蚌埠医学院学报, 2005, 30(2): 110-112.
    LU Zheng, ZHU Yan-Liang, HE Chang-Lin, LIU Mu-Lin, LIU Jin-Xin. Experimental study on the influence of spleen on the release of inflammatory mediator in rats with acute pancreatitis[J]. Journal of Bengbu Medical College, 2005, 30(2): 110-112.
    Citation: LU Zheng, ZHU Yan-Liang, HE Chang-Lin, LIU Mu-Lin, LIU Jin-Xin. Experimental study on the influence of spleen on the release of inflammatory mediator in rats with acute pancreatitis[J]. Journal of Bengbu Medical College, 2005, 30(2): 110-112.

    脾脏在大鼠急性胰腺炎炎症介质释放中的作用

    Experimental study on the influence of spleen on the release of inflammatory mediator in rats with acute pancreatitis

    • 摘要: 目的: 探讨脾脏在全身炎症反应综合征(SIRS)中对炎症介质释放的作用。方法: 大鼠胆胰管内注入3%牛磺胆酸钠(0.7ml/kg)及胰蛋白酶(3000u/kg)制成急性胰腺炎模型以引起SIRS。实验大鼠随机分成4组:假手术组,脾切除组,脾切除+急性胰腺炎组,急性胰腺炎组。分别观察各组血清淀粉酶、TNF-α、IL-1β、IL-6及IL-10水平,取末段回肠行光镜及透射电镜观察肠黏膜受损情况。结果: 脾切除+急性胰腺炎组TNF-α、IL-1β及IL-10均低于急性胰腺炎组(P<0.01);病理学检查显示,脾切除+急性胰腺炎组肠黏膜上皮仅轻微水肿,肠黏膜屏障基本完整,而急性胰腺炎组肠黏膜上皮水肿明显,绒毛坏死,上皮细胞变性,炎性细胞浸润。结论: 脾脏在急性炎症反应早期,可以明显促进炎症介质的产生和释放,加重炎症反应。脾脏切除后可减少促炎因子的产生和释放,肠黏膜屏障受损减轻。

       

      Abstract: Objective: To investigate the influence of spleen on the production and release of inflammatory mediator in acute pancreatitis.Methods: Acute necrotizing pancreatitis(ANP) was induced in SD rats by injecting 3% sodium taurocholate and trypsin into the biliopancreatic duct.All rats were divided into four groups randomly:sham operation group,splenectomy group,acute pareatitis with splenectomy group,and acute pancreatitis group.The serum levels of TNF-α,IL-1β,IL-6 and IL-10 in each group were examined.The terminal ileum was excised to observe the injury of intestinal mucosal barrier by optical microscope and electronic microscope.Results: The serum levels of TNF-α,IL-1β and IL-10 in the acute pareatitis with splenectomy group were significantly lower than those in the acute pancreatitis group(P<0.01).Histological examination revealed mild edema of mucosal epithelium and normally appearing intact mucosal barrier in the acute pareatitis with splenectomy group,whereas acute pancreatitis group revealed severe injuries including apparent edema,necrosis of the intestinal villi,degeneration of epithelial cell and infiltration of neutrophils.Conclusions: Spleen may promote the production and release of inflammatory mediators in acute inflammatory response,and aggravate the inflammatory response.Splenectomy can reduce the production and release of inflammatory mediators,thus decrease the damage of intestinal mucosal barrier.

       

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