Abstract: Objective: To explore the stereotactic anatomy of sylvian fissure based on the MRI,and its significance in brain stereotactic operation.Methods: The brain MRI images of 30 healthy people were collected and conversed.The values of X and Y-axis at each sample-point,which was located every 3 mm along the X direction of the outer edge of sylvian fissure to the inner side of insula were measured,and the Z value was the product of AC-PC layers and layer distance.The three-dimensional location data set of sylvian fissure were established by X,Y and Z-axis values.The coordinate values of each sample-point in the outer edge of sylvian fissure were analyzed to draw their projections in the sagittal and coronal plane.Results: The stereotactic data set of sylvian fissure was established successfully.The projection picture in the sagittal and coronal plane of sylvian fissure was successfully drawed,and its multiple linear regression equation was calculated.Conclusions: The stereotactic anatomy of sylvian fissure can provide the data for stereotactic surgery of brain,and establish brain atlas.