细胞化学染色在急性白血病分型中的应用

宫继勇; 毕早斌; 田万林

细胞化学染色在急性白血病分型中的应用

Application of cytochemistry staining in classification of acute leukemia

摘要

摘要: 目的: 探讨细胞化学染色法对急性白血病(acute leukemia,AL)诊断分型的价值。方法: 骨髓涂片常规瑞氏染色,细胞化学染色包括过氧化物酶(POX)、特异性酯酶(CE)、非特异性酯酶(NAE)(即α-醋酸萘酚酯)、糖原(PAS)(即过碘酸雪夫)染色。结果: POX、CE、PAS可区别急性淋巴细胞白血病(ALL)与急性非淋巴细胞白血病(ANLL),NAE加氟化钠抑制试验对区分粒系和单核系白血病有重要意义。细胞形态学对AL的确诊率为74.69%,加做细胞化学染色可将诊断率提高到90.1%。结论: 细胞化学染色对AL的确诊分型和鉴别诊断是不可缺少的客观指标,可以明显提高AL的诊断率,且方法稳定,操作简便。

Abstract: Objective: To diagnose and classify acute leukemia(AL)by cytochemistry staining.Methods: Bone marrow slides were stained with wright staining and cytochemistry staining including peroxidase(POX),naphthol AS-D chloroacetate esterase(CE),and alpha naphthyl-acetate esterase(NAE)with sodium fluoride inhibition and periodic acid Schiff reaction(PAS).Results: POX,CE and PAS could distinguish acute nonlymphocytic leukemia(ANLL)from acute lymphocytic leukemia(ALL).NAE with sodium fluoride inhibition could differentiate granulocytic leukemia from monocytic leukemia.Furthermore,the diagnostic rate of AL increased from 74.4% with only morphology to 90.1% with both cytochemistry and morphology.Conclusions: Cytochemistry staining is of great value for the diagnosis and classification of AL and can increase the conformity rate.

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