Abstract: Objective:To investigate the diagnostic significance of C-reactive protein (CRP) and procalcitonin (PCT) on sepsis. Methods:According to the criterion of sepsis compiled by America College of Chest Physicians/America Society of Critic Care Medicine in 1991. Sixty-four severe patients were selected in ICU. The patients were divided into two groups:sepsis group(n=50) and none sepsis group(n=14),the baseline clinical data of two groups were comparable. PCT and CRP were measured by double-antibody sandwich ELISA method, solid-phase semi-quantitative immunochromatography and quantitative immunization rate turbidimetry, the sensitivity,specificity,positive predictive value,negative predictive value,Youdens' index of two biomarkers were calculated. The ROC was described for further analysis. Results:The sensitivity, specificity, positive predictive value, negative predictive value, Youdens' index and the area under the curve of ROC of PCT were better than those of CRP. And the combination of two biomarkers had higher sensitivity than that of CRP or PCT separately. Conclusions:PCT has a relatively good diagnostic significance on sepsis. The combination of multibiomarkers should be considered to apply in order to increase the sensitivity of dignosis for sepsis.