Abstract: Objective: To study the bacterial distribution and drug resistance of pathogens in intensive care unit(ICU) and instruct clinical application of antibiotics reasonably. Methods: Pathogenic bacteria isolated from sputum and blood in all patients in ICU of our hospital from August 2007 to August 2009 were identified and drug sensitivity test was performed. Results: A total of 112 strains bacteria were collected. 84 gram-negative bacilli,14 gram-positive cocci and 14 fungi,which accounted for 75.0%,12.5% and 12.5% respectively. The drug sensitivity test included that the drug resistance in pseudomonas aeruginosa and acinetobacter baumanni were serious,all the enterobacteriaceae bacteria were sensitive to imipenem, gram-positive cocci were sensitive to vancomycin, both of staphylococci and enterococci were sensitive to vancomycin. Conclusions: The pathogens of ICU are mainly isolated from respiratory tract,most of them are gram-negative bacilli and multi-drug-resistant. The detection of pathogens and drug resistance of them should be emphasized in ICU,which could be helpful to clinical application of antibiotics reasonably and control infection.