高级检索

    猪囊尾蚴病患者外周血T细胞亚群比例和产生IFN-γ、IL-4水平研究

    Study on the proportion of peripheval blood T cell subsets and the level of IFN-γ and IL-4 production in patients with cysticercosis

      摘要
    • 摘要: 目的:检测猪囊尾蚴病患者外周血T细胞(peripheral blood mononuclear cell,PBMC)体外诱生IFN-γ、IL-4的水平,探讨T细胞亚群在感染免疫中的调控。方法:采用流式细胞仪检测猪囊尾蚴病患者PBMC中T细胞亚群比例和IFN-γ/IL-4水平;用Cellquest软件或WinMDI2.8软件数据分析。结果:猪囊尾蚴病患者T细胞亚群CD3+与CD4+细胞百分率均较正常人升高(P<0.01),CD4+/CD8+比值亦较正常人升高(P<0.01);PBMC中分泌IFN-γ及IL-4的CD3+细胞百分率与正常人差异均无统计学意义(P>0.05)。结论:猪囊尾蚴病患者PBMC中CD3+、CD4+细胞百分率显著升高,T细胞亚群比例失调,免疫功能紊乱。

       

      Abstract: Objective: To detect the proportion of peripheval blood T cell subsets(PBMCs) and the level of IFN-γ/IL-4 production of peripheral blood in patients with cysticercosis,and to investigate the role of T cell subsets in immune regulation of cysticercus cellulosae infection.Methods: The proportions of T-cell subsets of PBMCs and Th1/Th2 cytokines producing cells in patients with cysticercosis were detected by four-color flow cytometry.Cellquest or WinMDI 2.8 was used in analysis of T-cell subsets.Results: The percentage of CD3+ and CD4+ T-cells subsets,and the ratio of CD4+/CD8 in patients with cysticercosis were higher than those of the control(P<0.01).The percentage of secreting IFN-γ/IL-4 CD3+ of PBMC in patients with cysticercosis was not higher than that of the control(P>0.05).Conclusions: The patients with cysticercosis have the higher percentages of CD3+ and CD4+ cells than the control,presenting an imbalance of T cell subsets and abnormality of immunity.

       

      • HTML全文
      • 参考文献(0)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回