Abstract: Objective: application of WS/T403-2012 standards of evaluation and biochemical status of quality control.Method:WS/T403-2012, 23 indexes according to the index grade is divided into good, medium, low and less than four low, with each of the biological variation in the quality index of the best, Hope, minimum total allowable error close. Below the low according to current quality level setting quality objectives. The above items are respectively matched comparison analysis. Results: in 21 routine examination in the health ministry EQA of creatine kinase, only the sigma 6 , without any of the improvement measures; K+, Na+, Ca2+ transfer, Mg2+, uric acid, creatinine, cholesterol, triglyceride, three alanine aminotransferase, aspartate aminotransferase aminotransferase, total bilirubin, alkaline phosphatase, amylase, lactic dehydrogenase, gamma glutamyl transpeptidase, still can reach the standard WS/T403-2012 quality level required; and urea nitrogen, glucose, total protein, Bai Dan white, Cl- quality state is currently not ideal. Conclusion: for quality state owed the ideal project needs to take the comprehensive quality control strategy, and improve the accuracy and / or precision, ensure the quality control level to meet the requirements