Abstract: Objective:To explore the value of human epididymis protein 4(HE4) in diagnosis of ovarian cancer in different clinical stages,and determine whether HE4 is better than cancer antigen 125(CA125),another tumor marker of ovarian cancer. Methods:The serum HE4 levels in 46 patients with ovarian cancer(among them,the numbers of early,intermediate and terminal cancer were 9,31 and 6,respectively), 50 patients with benign ovarian tumour and 50 healthy women were measured by automatic chemiluminescence method. Results:The level of serum HE4 was significantly higher in ovarian cancer patients than those in other two groups(P<0.01). In 9 early ovarian cancer patients,the positive rate of HE4 was 78%,which was significantly higher than CA125,the positive rate was 55%. Conclusions:HE4 is a specific marker and it is better than CA125 in diagnosis of ovarian cancer,especially it is important for diagnosis of early ovarian cancer.