乙酰肝素酶和syndecan-1表达与脑胶质瘤恶性程度的关系
The relationship of the expression of heparanse and syndecan-1 in glioma tissues to the grades
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摘要: 目的:检测脑胶质瘤组织中乙酰肝素酶和syndecan-1的表达水平,探讨它们和肿瘤级别的关系及作用机制。方法:采用荧光实时定量聚合酶链式反应(RT-PCR)法检测乙酰肝素酶mRNA和syndecan-1 mRNA在40例脑胶质瘤组织和10例良性脑肿瘤组织中的表达水平,统计分析表达水平和肿瘤级别之间的关系。结果:在胶质瘤中,乙酰肝素酶的表达明显高于良性脑肿瘤(P<0.01),并且随着肿瘤的恶性程度增高,乙酰肝素酶的表达也明显增高(P<0.01),syndecan-1的表达明显低于良性脑肿瘤(P<0.01),并且随着肿瘤的恶性程度增高,syndecan-1的表达也明显降低(P<0.01)。乙酰肝素酶与syndecan-1在Ⅰ~Ⅱ级脑胶质瘤中表达均呈负相关关系(P<0.01),在Ⅲ~Ⅳ级脑胶质瘤中表达均呈负相关关系(P<0.01),在各级胶质瘤中表达相关性的总体分析均显示负相关关系(P<0.01)。结论:乙酰肝素酶在脑胶质瘤组织中高表达,syndecan-1在脑胶质瘤中低表达,而且表达水平和恶性程度密切相关,它们的检测可以作为脑胶质瘤恶性程度判断的参考,为探讨脑胶质瘤的生物学行为、预后及治疗提供新的思路。Abstract: Objective:To investigate the reltationship between the expression of heparanase and syndecan-1 gene mRNA and the pathological grade in human gliomas. Methods:The realtime polymerase chain reactions was empolyed to detect the expression level of heparanase and syndecan-1 gene mRNA in 40 cases of human gliomas and in 10 cases of benign brain tumors,which was used to evaluate tumor grade. Results:The expression level of heparanase mRNA in gliomas is higher than that in brain tumors(P<0.01),and rising with tumor grade. The expression level of syndecan-1 mRNA in gliomas is significant lower compared to that of benign brain tumors(P<0.01),and decresing with tumor grade. The expression level of heparanase mRNA is reversely correlated to the expression level of syndecan-1 mRNA in human gliomas(P<0.01). Conclusions:The heparanase and syndecan-1 gene expression maybe correlate to the oncogensis and progression of human gliomas,which can provide an effective way to diagnose and treat the human gliomas.
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