Abstract: Objective:To construct a kind of extracellular matrix scaffold and investigate whether it can influence the expression of neurotrophins in rat olfactory ensheathing cells.Methods:Mixed fibrinogen,laminin and fibronectin proportionally,then added fresh rat plasma to construct scaffold.Plated the rat olfactory ensheathing cells (OEC) on scaffold and then observed the influence of scaffold on proliferation and neurotrophin expression of OEC.Results:Rat OEC adhered to scaffold easily,proliferated quickly and extended long processes.Immunofluorescence and western blotting showed rat OEC on scaffold could produce more neurotrophins than those on glass.Conclusions:Extracellular matrix scaffold can promote proliferation of rat OEC and promote the production of neurotrophins.