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    己烯雌酚对胎鼠海马神经干细胞增殖与分化的影响

    Influence of diethylstilbestrol on proliferation and differentiation of hippocampal neural stem cells

      摘要
    • 摘要: 目的:探讨己烯雌酚对胎鼠海马神经干细胞(neural stem cells,NSCs)的促增殖、分化作用及其机制。方法:取孕16天SD胎鼠海马NSCs培养,分别设空白对照组,己烯雌酚1×10-7 mol/L组、1×10-8 mol/L组、1×10-9 mol/L组,以及脑源性神经营养因子(BDNF)5×10-2 mg/L组;免疫荧光化学方法检测Nestin、神经元特异烯醇化酶及神经胶质酸性蛋白的表达,RT-PCR检测BDNF mRNA的含量。结果:不同剂量己烯雌酚处理各组神经球面积、积分光密度值、平均突起长度与平均突起数均增加,其中10-8 mol/L组海马NSC增殖与分化最明显,BDNF mRNA表达量亦最高(P<0.01)。结论:己烯雌酚对海马NSCs的增殖分化具有促进作用,且可能与上调BDNF的表达有关。

       

      Abstract: Objective: To investigate the mechanism of the effect of diethylstilbestrol on the proliferation and differentiation of hippocampal neural stem cells(NSCs) in fetal rats.Methods: Hippocampal neural stem cells from the embryo(gestational day 16) of fetal rats were prepared and divided into five groups:control,diethylstilbestrol 1×10-7 mol/L,diethylstilbestrol 1×10-8 mol/L,diethylstilbestrol 1×10-9 mol/L and BDNF 5×10-2 mg/L.The expressions of Nestin,neurone specific enolase(NSE) and glial fibrillary acidic protein(GFAP) were determined by immunohistochemistry,and the level of brain-derived neurotrophic factor(BDNF) mRNA was detected by RT-PCR.Results: Different doses of diethylstilbestrol could increase the area of neural spheres,quantity of NSE,and GFAP-positive cells.At a concentration of 10-8 mol/L,diethylstilbestrol facilitated the proliferation and differentiation of hippocampal NSCs of fetal rats the most,and the level of BDNA mRNA was the highest(P<0.01).Conclusions: Diethylstilbestrol enhances the proliferation of hippocampal NSCs in fetal rats,which is probably related to the up-regulation of the BDNF mRNAs' expression.

       

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