Abstract: Objective: To monitor the composition and drug resistance of the pathogenic bacteria isolated from the urine of patients with urinary tract infections.Methods: One thousand one hundred and twenty-six drug-resistant bacteria isolated from urine were identified and analyzed,and the special drug-resistant strains were detected by K-B method.The sensitivity rate,intermediate rate and drug resistance rate were determined in accordance with CLSI 2006 standard.Results: Of the 1 126 strains isolated from patients with urinary tract infection,E.coli-based strains accounted for 45.5% (512/1 126),coagulase-negative Staphylococci 18.5% (208/1 126),Enterococcus 9.9% (112/1 126),Candida albicans 5.4% (61/1 126),Mycetozoan 4.9% (55/1 126),Klebsiella pneumoniae 4.6% (52/1 126),and the others were Enterobacter cloacae and Pseudomonas aeruginosa (Corynebacterium 4,such as Acinetobacter baumannii).The detection rate of ESBLs was 48.0% in E.coli,38.6% in Klebsiella pneumoniae,9.5% in Pseudomonas aeruginosa,78.5% in methicillin-resistant staphylococcus aureus,1.8% of vancomycin resistant enterococcal and 80.4% of high level aminoglikozyde resistance.Only 1.4% of Ampc was detected in E.coli strains.Carbapenems was still the most effective antibiotics for gram-negative bacteria (except maltophilia S.m and Flavobacterium).Cefoperazone/sulbactam,piperacillin/tazobactam and cefepime also presented good antibacterial activity.The resistance rate of Gram-positive bacteria was relatively high,only maintaining good sensitivity to vancomycin.Conclusions: Enterobacteriaceae and coagulase-negative staphylococci are the main pathogens for urinary tract infections.The resistant rate of a variety of pathogens tends to rise.Carbon penicillins and vancomycin are the best and strongest antibiotics for gram-negative bacilli and gram-positive cocci respectively,which is worth clinical attention.