标本因素对荧光定量PCR检测HBV DNA的影响

Sample factors affecting fluorescence quantitative PCR detection of HBVDNA

摘要: 目的: 探讨标本溶血、黄疸、脂血对荧光定量PCR检测乙型肝炎病毒(HBV)DNA的影响。方法: 荧光定量PCR检测无溶血、无黄疸、非脂血血清和溶血、黄疸、高脂血清标本的HBV DNA。结果: 溶血、黄疸、高脂血血清与无溶血、无黄疸、非脂血血清HBV DNA定量检测结果差异均无统计学意义(P>0.05)。结论: 标本溶血、黄疸、脂血对荧光定量PCR检测HBV DNA无干扰。

Abstract: Objective: To explore the effects of abnormal sample characters,such as hemolysis,icterus and lipemia,on fluorescence quantitative PCR (FQ-PCR) detection of HBV DNA. Methods: Levels of serum HBV DNA in hyperlipemia with hemolysis,icterus and normal serum samples were detected by FQ-PCR. Results: There were no significant differences of HBV DNA level among the groups of hyperlipemia with hemolysis,icterus and the control (P>0.05). Conclusions: Hemolysis,icterus and lipemia of serum sample have no interference on FQ-PCR detection of HBV DNA.