Abstract: Objective: To determine the clinical value of the tumor marker squamous cell carcinoma antigen(SCCAg) in the diagnosis of cervical squamous cell carcinoma and to validate the sensitivity and specificity of chemiluminescence detection of SCCAg. Methods: The serum SCCAg levels in one hundred and forty cervical squamous carcinoma patients and one hundred and fifty healthy women controls were measured by ARCHITECT I2000SR automatic immuno-analyzer. Results: The levels of SCCAg in cervical squamous carcinoma patients and normal controls were (21.54 ±39.95) ng/ml and (0.69 ±0.42) ng/ml, respectively. There was a statistically significant difference between the two groups(P < 0.01). The positive rate of cervical squamous carcinoma patients was 86.4% (121/140) when > x+3s of normal control group(> 1.9 ng/ml) was used as a positive standard. Conclusions: SCCAg can be used as a specific marker in the diagnosis of cervical squamous cell carcinoma. It is a rapid, simple, accurate and specific method, which is also easy to be accepted by patients. Thus,the detection of SCCAg has a very important significance for the curative effect observation and the prognosis evaluation of cervical squamous cell carcinoma.