异丙肾上腺素诱导慢性心力衰竭大鼠模型的建立

    Establishment of model of isoprenaline-induced chronic heart failure in rats

    • 摘要: 目的:建立异丙肾上腺素(isoprenaline,ISO)诱导SD大鼠慢性心力衰竭(CHF)模型,并对其进行评估。方法:用ISO (170 mg/kg)给SD大鼠皮下注射2次,建立大鼠CHF模型,分别在用药后3、6、18周进行心脏超声检测左室舒张末内径(LVEDD)、左室收缩末内径(LVESD)、左室射血分数(LVEF)、缩短分数(FS),18周取心肌观察病理形态学特征。结果:随着时间的延长,大鼠CHF程度逐渐加重,病死率增加;随着LVEF和FS下降,LVEDD、LVESD扩大(P<0.01),左室重量指数明显增加(P<0.01)。结论:大剂量ISO诱导建立大鼠CHF模型的方法简便、可靠;超声心动图检测对于大鼠CHF模型的建立与评估具有指导意义。

       

      Abstract: Objective: To establish the model of isoprenaline (ISO)-induced chronic heart failure(CHF) in rats and to assess the value.Methods: The ISO-induced group(ISO group,n=50) received two subcutaneous injections of 170 mg ISO per kilogram of body and the healthy control group(NS group,n=10) received two subcutaneous injections of 0.25 ml normal saline.The left ventricular end-diastolic dimension(LVEDD),left ventricualr end-systolic dimension(LVESD),left ventricular ejection fraction(LVEF) and shortening fractionl(FS) were detected by echocardiogram at 3,6 and 18 weeks after the injection.Eighteen weeks later,the mass ratio of heart ventricle and left ventricle were measured.Results: As time went on,the degree of CHF became more serious and there was an increase of mortality(P<0.01).Along with the decrease of LVEF and FS,LVEDD and LVESD increased significantly(P<0.01);the mass ratio of heart ventricle and left ventricle increased significantly(P<0.01).Conclusions: High-dose of ISO-induction is a simple,convenient and reliable method to trigger the rat model of CHF.Echocardiographic assessment is instructive in this species.

       

    /

    返回文章
    返回